Andrea N. Crivaro , Paula Carasi , Ileana Salto , Ayelen Hugo , P. Cecilia Soldavini Pelichotti , Agustina Bengoa , Melisa Fragomeno , María A. Serradell , Jessica Minnaard , Ivanna Rolny , Eduardo Alul , Leandro Arregui , Macarena E. Fabra Martinez , Oscar Javier Moreno Valero , Andrea Facente , Francisco Magariños , Virginia Jewtuchowicz , Pablo F. Pérez , Fernando M. Trejo
{"title":"艰难梭菌:阿根廷一家公立医院循环毒素型的特征","authors":"Andrea N. Crivaro , Paula Carasi , Ileana Salto , Ayelen Hugo , P. Cecilia Soldavini Pelichotti , Agustina Bengoa , Melisa Fragomeno , María A. Serradell , Jessica Minnaard , Ivanna Rolny , Eduardo Alul , Leandro Arregui , Macarena E. Fabra Martinez , Oscar Javier Moreno Valero , Andrea Facente , Francisco Magariños , Virginia Jewtuchowicz , Pablo F. Pérez , Fernando M. Trejo","doi":"10.1016/j.ram.2022.05.010","DOIUrl":null,"url":null,"abstract":"<div><p><em>Clostridioides difficile</em> is a spore-forming anaerobe microorganism associated to nosocomial diarrhea. Its virulence is mainly associated with TcdA and TcdB toxins, encoded by their respective <em>tcdA</em> and <em>tcdB</em> genes. These genes are part of the pathogenicity locus (PaLoc). Our aim was to characterize relevant <em>C. difficile</em> toxinotypes circulating in the hospital setting. The <em>tcdA</em> and <em>tcdB</em> genes were amplified and digested with different restriction enzymes: EcoRI for <em>tcdA</em>; HincII and AccI for <em>tcdB</em>. In addition, the presence of the <em>cdtB</em> (binary toxin) gene, TcdA and TcdB toxins by dot blot and the cytotoxic effect of culture supernatants on Vero cells, were evaluated. Altogether, these studies revealed three different circulating toxinotypes according to Rupnik's classification: 0, I and VIII, being the latter the most prevalent one. Even though more studies are certainly necessary (e.g. sequencing analysis), it is worth noting that the occurrence of toxinotype I could be related to the introduction of bacteria from different geographical origins.</p><p>The multivariate analysis conducted on the laboratory values of individuals infected with the most prevalent toxinotype (VIII) showed that the isolates associated with fatal outcomes (GCD13, GCD14 and GCD22) are located in regions of the biplots related to altered laboratory values at admission.</p><p>In other patients, although laboratory values at admission were not correlated, levels of urea, creatinine and white blood cells were positively correlated after the infection was diagnosed.</p><p>Our study reveals the circulation of different toxinotypes of <em>C. difficile</em> strains in this public hospital. The variety of toxinotypes can arise from pre-existing microorganisms as well as through the introduction of bacteria from other geographical regions. The existence of microorganisms with different pathogenic potential is relevant for the control, follow-up, and treatment of the infections.</p></div>","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Clostridioides difficile: Characterization of the circulating toxinotypes in an Argentinean public hospital\",\"authors\":\"Andrea N. Crivaro , Paula Carasi , Ileana Salto , Ayelen Hugo , P. Cecilia Soldavini Pelichotti , Agustina Bengoa , Melisa Fragomeno , María A. Serradell , Jessica Minnaard , Ivanna Rolny , Eduardo Alul , Leandro Arregui , Macarena E. Fabra Martinez , Oscar Javier Moreno Valero , Andrea Facente , Francisco Magariños , Virginia Jewtuchowicz , Pablo F. Pérez , Fernando M. Trejo\",\"doi\":\"10.1016/j.ram.2022.05.010\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><em>Clostridioides difficile</em> is a spore-forming anaerobe microorganism associated to nosocomial diarrhea. Its virulence is mainly associated with TcdA and TcdB toxins, encoded by their respective <em>tcdA</em> and <em>tcdB</em> genes. These genes are part of the pathogenicity locus (PaLoc). Our aim was to characterize relevant <em>C. difficile</em> toxinotypes circulating in the hospital setting. The <em>tcdA</em> and <em>tcdB</em> genes were amplified and digested with different restriction enzymes: EcoRI for <em>tcdA</em>; HincII and AccI for <em>tcdB</em>. In addition, the presence of the <em>cdtB</em> (binary toxin) gene, TcdA and TcdB toxins by dot blot and the cytotoxic effect of culture supernatants on Vero cells, were evaluated. Altogether, these studies revealed three different circulating toxinotypes according to Rupnik's classification: 0, I and VIII, being the latter the most prevalent one. Even though more studies are certainly necessary (e.g. sequencing analysis), it is worth noting that the occurrence of toxinotype I could be related to the introduction of bacteria from different geographical origins.</p><p>The multivariate analysis conducted on the laboratory values of individuals infected with the most prevalent toxinotype (VIII) showed that the isolates associated with fatal outcomes (GCD13, GCD14 and GCD22) are located in regions of the biplots related to altered laboratory values at admission.</p><p>In other patients, although laboratory values at admission were not correlated, levels of urea, creatinine and white blood cells were positively correlated after the infection was diagnosed.</p><p>Our study reveals the circulation of different toxinotypes of <em>C. difficile</em> strains in this public hospital. The variety of toxinotypes can arise from pre-existing microorganisms as well as through the introduction of bacteria from other geographical regions. The existence of microorganisms with different pathogenic potential is relevant for the control, follow-up, and treatment of the infections.</p></div>\",\"PeriodicalId\":1,\"journal\":{\"name\":\"Accounts of Chemical Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":16.4000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Accounts of Chemical Research\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0325754122000554\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0325754122000554","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Clostridioides difficile: Characterization of the circulating toxinotypes in an Argentinean public hospital
Clostridioides difficile is a spore-forming anaerobe microorganism associated to nosocomial diarrhea. Its virulence is mainly associated with TcdA and TcdB toxins, encoded by their respective tcdA and tcdB genes. These genes are part of the pathogenicity locus (PaLoc). Our aim was to characterize relevant C. difficile toxinotypes circulating in the hospital setting. The tcdA and tcdB genes were amplified and digested with different restriction enzymes: EcoRI for tcdA; HincII and AccI for tcdB. In addition, the presence of the cdtB (binary toxin) gene, TcdA and TcdB toxins by dot blot and the cytotoxic effect of culture supernatants on Vero cells, were evaluated. Altogether, these studies revealed three different circulating toxinotypes according to Rupnik's classification: 0, I and VIII, being the latter the most prevalent one. Even though more studies are certainly necessary (e.g. sequencing analysis), it is worth noting that the occurrence of toxinotype I could be related to the introduction of bacteria from different geographical origins.
The multivariate analysis conducted on the laboratory values of individuals infected with the most prevalent toxinotype (VIII) showed that the isolates associated with fatal outcomes (GCD13, GCD14 and GCD22) are located in regions of the biplots related to altered laboratory values at admission.
In other patients, although laboratory values at admission were not correlated, levels of urea, creatinine and white blood cells were positively correlated after the infection was diagnosed.
Our study reveals the circulation of different toxinotypes of C. difficile strains in this public hospital. The variety of toxinotypes can arise from pre-existing microorganisms as well as through the introduction of bacteria from other geographical regions. The existence of microorganisms with different pathogenic potential is relevant for the control, follow-up, and treatment of the infections.
期刊介绍：
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
