GPRC5B (G蛋白偶联受体C类5组成员B)抑制葡萄糖饥饿诱导的头颈部鳞状细胞癌细胞凋亡。

IF 1.3 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
Keisuke Kanamori, Shigeyuki Ozawa, Hiroshi Iwabuchi, Takeharu Ikoma, Kenji Suzuki, Kae Tanaka, Natsuko Yoshimura Sawai, Takahiro Abe, Yasumasa Kato, Ryu-Ichiro Hata, Masaru Kobayashi
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引用次数: 0

摘要

G蛋白偶联受体C类5组成员B (GPRC5B)参与细胞外葡萄糖感知、葡萄糖代谢和胰岛素抵抗。许多癌症需要高浓度的葡萄糖才能存活和生长。我们利用来自the Human Protein Atlas的数据,研究了肿瘤GPRC5B表达与包括头颈部鳞状细胞癌(HNSCC)在内的癌症患者预后之间的关系。在HNSCC、胃癌、胰腺癌、结直肠癌和乳腺癌患者中,如果其肿瘤表现出高水平的GPRC5B表达,则其5年生存率显著降低。GPRC5B在葡萄糖代谢中的作用通过6个不同水平GPRC5B表达的HNSCC细胞系进行评估。高水平的GPRC5B表达有利于细胞的快速生长。对正常表达和转染GPRC5B的HNSCC细胞系的生存能力进行了评估,在标准培养条件下没有观察到差异。然而,在葡萄糖缺乏的培养条件下,过表达gprc5b的细胞表现出更高的活力和更少的凋亡。研究结果强调了GPRC5B高表达与包括HNSCC在内的各种癌症患者5年生存率差之间的关联。此外,我们已经证明GPRC5B在葡萄糖消耗条件下支持癌细胞存活,并可能成为癌症治疗的靶分子。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
GPRC5B (G protein-coupled receptor class C group 5 member B) suppresses glucose starvation-induced apoptosis in head-and-neck squamous cell carcinoma.

G protein-coupled receptor class C group 5 member B (GPRC5B) is involved in extracellular glucose sensing, glucose metabolism, and insulin resistance. Many cancers require glucose at high concentrations to survive and grow. We have investigated the association between tumour GPRC5B expression and the prognosis for patients with cancer, including head-and-neck squamous cell carcinoma (HNSCC), using data from The Human Protein Atlas. The 5-year survival rate was significantly reduced in patients with HNSCC, gastric, pancreatic, colorectal, and breast cancers if their tumours exhibited high levels of GPRC5B expression. The role of GPRC5B in glucose metabolism was assessed using six HNSCC cell lines with varying levels of GPRC5B expression. High levels of GPRC5B expression were found to favour rapid cell growth. The viability of an HNSCC cell line with normal and transfected GPRC5B expression was also assessed and no differences were observed under standard culture conditions. However, under glucose-deficient culture conditions, GPRC5B-overexpressing cells exhibited increased viability and reduced apoptosis. The results highlight the association between high GPRC5B expression and poor 5-year survival rates in patients with various cancers, including HNSCC. Furthermore, we have demonstrated that GPRC5B supports cancer cell survival under glucose-depleted conditions and could be a target molecule for cancer therapy.

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来源期刊
Biomedical Research-tokyo
Biomedical Research-tokyo 医学-医学:研究与实验
CiteScore
2.40
自引率
0.00%
发文量
19
审稿时长
>12 weeks
期刊介绍: Biomedical Research is peer-reviewed International Research Journal . It was first launched in 1990 as a biannual English Journal and later became triannual. From 2008 it is published in Jan-Apr/ May-Aug/ Sep-Dec..
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