变应性炎症通过小鼠结肠胆碱能调节破坏上皮电致电解质运输。

IF 1.3 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
Takeshi Yamamoto, Yosuke Katsuki, Yuya Kanauchi, Shusaku Hayashi, Makoto Kadowaki
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引用次数: 0

摘要

电解质的肠内转运受肠神经系统的调节。乙酰胆碱(ACh)被认为是结肠电解质运输中最重要的神经递质。然而,在结肠中由乙酰胆碱调节的电解质转运尚不完全清楚。本研究通过Ussing室系统测量短路电流(Isc),研究了胆碱能激动剂对小鼠结肠电致电解质运输的调节作用。毒蕈碱刺激可诱导正常小鼠结肠瞬时电原性Cl-分泌,烟碱刺激可诱导电原性K+分泌至结肠顶侧,食物过敏小鼠的上述作用减弱。强的松龙对FA小鼠的治疗抑制了结肠轻度炎症和过敏症状,从而改善了胆碱能通路激活、电场刺激和结肠细胞内cAMP信号通路激活所引起的电致电解质运输的中断。这些结果表明,FA诱导的结肠炎症损害了结肠的电原电解质运输功能,抑制炎症可改善FA小鼠结肠的电原电解质运输功能障碍。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Allergic inflammation disrupts epithelial electrogenic electrolyte transport through cholinergic regulation in the mouse colon.

Intestinal transport of electrolytes is regulated by the enteric nervous system. Acetylcholine (ACh) is considered the most important neurotransmitter for electrolyte transport in the colon. However, electrolyte transport regulated by ACh is not fully understood in the colon. We investigated the regulation of electrogenic electrolyte transport by cholinergic agonists in the mouse colon by measuring the short-circuit current (Isc) using an Ussing chamber system. Muscarinic stimulation induced transient electrogenic Cl- secretion, and nicotinic stimulation induced electrogenic K+ secretion to the apical side in the normal mouse colon, and these effects were reduced in the colon of mice with food allergy (FA). Administration of prednisolone to mice with FA suppressed mild inflammation in the colon and allergic symptoms and thereby ameliorated the disruption of electrogenic electrolyte transport induced not only by cholinergic pathway activation but also by electrical field stimulation and intracellular cAMP signaling pathway activation in the colon. These results suggest that the electrogenic electrolyte transport function in the colon is impaired by FA-induced colonic inflammation and that the suppression of inflammation ameliorates the dysfunction of electrogenic electrolyte transport in the colon of mice with FA.

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来源期刊
Biomedical Research-tokyo
Biomedical Research-tokyo 医学-医学:研究与实验
CiteScore
2.40
自引率
0.00%
发文量
19
审稿时长
>12 weeks
期刊介绍: Biomedical Research is peer-reviewed International Research Journal . It was first launched in 1990 as a biannual English Journal and later became triannual. From 2008 it is published in Jan-Apr/ May-Aug/ Sep-Dec..
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