E. J. Baldassarri, M. G. Ortore, C. Ferrero, S. Finet, F. Spinozzi, P. Mariani
{"title":"两种不同反离子稳定g -四元结构的压力效应","authors":"E. J. Baldassarri, M. G. Ortore, C. Ferrero, S. Finet, F. Spinozzi, P. Mariani","doi":"10.15866/IREBIC.V2I4.1526","DOIUrl":null,"url":null,"abstract":"It is well known that the formation of G-quadruplex by guanosine derivatives strongly depends on counter-ions. To investigate quadruplex stability in the presence of different counter-ions and in the absence of a covalent axial backbone, high-pressure X-ray diffraction experiments have been performed on lyotropic phases of guanosine 5’-monophosphate (GMP) in the form of sodium and ammonium salts. As a result, concentration-pressure phase diagrams were obtained in a pressure range from 1 bar to about 2 kbar, and the structural properties of the different phases derived. Interestingly, cholesteric (Ch) and hexagonal (H) columnar phases were found in both GMP salts, but during compression a reverse behavior was detected: in GMP sodium salt, pressure induces a H-Ch phase transition, while in GMP ammonium salt the induced transition is Ch-H. Different counter-ion stabilization, which control quadruplex length and the consequence of lateral interactions, probably explain such observation","PeriodicalId":14377,"journal":{"name":"International Review of Biophysical Chemistry","volume":"67 4","pages":"142-146"},"PeriodicalIF":0.0000,"publicationDate":"2011-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Pressure Effects on G-Quadruplex Structures Stabilized by Two Different Counter-Ions\",\"authors\":\"E. J. Baldassarri, M. G. Ortore, C. Ferrero, S. Finet, F. Spinozzi, P. Mariani\",\"doi\":\"10.15866/IREBIC.V2I4.1526\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"It is well known that the formation of G-quadruplex by guanosine derivatives strongly depends on counter-ions. To investigate quadruplex stability in the presence of different counter-ions and in the absence of a covalent axial backbone, high-pressure X-ray diffraction experiments have been performed on lyotropic phases of guanosine 5’-monophosphate (GMP) in the form of sodium and ammonium salts. As a result, concentration-pressure phase diagrams were obtained in a pressure range from 1 bar to about 2 kbar, and the structural properties of the different phases derived. Interestingly, cholesteric (Ch) and hexagonal (H) columnar phases were found in both GMP salts, but during compression a reverse behavior was detected: in GMP sodium salt, pressure induces a H-Ch phase transition, while in GMP ammonium salt the induced transition is Ch-H. Different counter-ion stabilization, which control quadruplex length and the consequence of lateral interactions, probably explain such observation\",\"PeriodicalId\":14377,\"journal\":{\"name\":\"International Review of Biophysical Chemistry\",\"volume\":\"67 4\",\"pages\":\"142-146\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2011-08-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Review of Biophysical Chemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15866/IREBIC.V2I4.1526\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Review of Biophysical Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15866/IREBIC.V2I4.1526","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Pressure Effects on G-Quadruplex Structures Stabilized by Two Different Counter-Ions
It is well known that the formation of G-quadruplex by guanosine derivatives strongly depends on counter-ions. To investigate quadruplex stability in the presence of different counter-ions and in the absence of a covalent axial backbone, high-pressure X-ray diffraction experiments have been performed on lyotropic phases of guanosine 5’-monophosphate (GMP) in the form of sodium and ammonium salts. As a result, concentration-pressure phase diagrams were obtained in a pressure range from 1 bar to about 2 kbar, and the structural properties of the different phases derived. Interestingly, cholesteric (Ch) and hexagonal (H) columnar phases were found in both GMP salts, but during compression a reverse behavior was detected: in GMP sodium salt, pressure induces a H-Ch phase transition, while in GMP ammonium salt the induced transition is Ch-H. Different counter-ion stabilization, which control quadruplex length and the consequence of lateral interactions, probably explain such observation