{"title":"可以直接病毒抗体检测","authors":"Gabriele Döller , Josef Jakubik","doi":"10.1016/S0172-5599(80)80014-9","DOIUrl":null,"url":null,"abstract":"<div><p>A direct solid-phase radioimmunoassay (dRIA) was developed in order to demonstrate antibodies against Aujeszky's disease virus (ADV) in sera obtained from pigs and rabbits. In the presence of guinea-pig complement the above test is 160-fold to 1500-fold more sensitive than the neutralization test (NT) and 320-fold to 150000-fold more sensitive than NT without complement. By means of dRIA ADV antibodies could be detected in swine sera obtained from an ADV-infected farm, which were found to be negative in the complement assisted NT. It is possible to test a single dilution of unknown serum by dRIA by comparing same with a standard curve and to make a statement regarding its ADV-specific binding capacity to <sup>125</sup>I-labelled ADV antigen.</p><p>The advantages of dRIA in comparison to the indirect RIA and the advantages and disadvantages with regard to ELISA were discussed.</p></div>","PeriodicalId":101293,"journal":{"name":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","volume":"247 1","pages":"Pages 1-7"},"PeriodicalIF":0.0000,"publicationDate":"1980-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80014-9","citationCount":"5","resultStr":"{\"title\":\"Direkter Festphasen-Radioimmunoassay zum Nachweis von Aujeszkyvirus-Antikörpern\",\"authors\":\"Gabriele Döller , Josef Jakubik\",\"doi\":\"10.1016/S0172-5599(80)80014-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A direct solid-phase radioimmunoassay (dRIA) was developed in order to demonstrate antibodies against Aujeszky's disease virus (ADV) in sera obtained from pigs and rabbits. In the presence of guinea-pig complement the above test is 160-fold to 1500-fold more sensitive than the neutralization test (NT) and 320-fold to 150000-fold more sensitive than NT without complement. By means of dRIA ADV antibodies could be detected in swine sera obtained from an ADV-infected farm, which were found to be negative in the complement assisted NT. It is possible to test a single dilution of unknown serum by dRIA by comparing same with a standard curve and to make a statement regarding its ADV-specific binding capacity to <sup>125</sup>I-labelled ADV antigen.</p><p>The advantages of dRIA in comparison to the indirect RIA and the advantages and disadvantages with regard to ELISA were discussed.</p></div>\",\"PeriodicalId\":101293,\"journal\":{\"name\":\"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie\",\"volume\":\"247 1\",\"pages\":\"Pages 1-7\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1980-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0172-5599(80)80014-9\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0172559980800149\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0172559980800149","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Direkter Festphasen-Radioimmunoassay zum Nachweis von Aujeszkyvirus-Antikörpern
A direct solid-phase radioimmunoassay (dRIA) was developed in order to demonstrate antibodies against Aujeszky's disease virus (ADV) in sera obtained from pigs and rabbits. In the presence of guinea-pig complement the above test is 160-fold to 1500-fold more sensitive than the neutralization test (NT) and 320-fold to 150000-fold more sensitive than NT without complement. By means of dRIA ADV antibodies could be detected in swine sera obtained from an ADV-infected farm, which were found to be negative in the complement assisted NT. It is possible to test a single dilution of unknown serum by dRIA by comparing same with a standard curve and to make a statement regarding its ADV-specific binding capacity to 125I-labelled ADV antigen.
The advantages of dRIA in comparison to the indirect RIA and the advantages and disadvantages with regard to ELISA were discussed.
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