木贼草提取物总黄酮体外细胞毒活性研究。

IF 1.6 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Raghda Makia, Khulood Al-Sammarrae, Mohammad Al-Halbosiy, Mohammed Al-Mashhadani
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引用次数: 2

摘要

背景:黄酮类化合物等正常发生的物质被认为是治疗和预防癌症的有效候选物质,本研究的目的是观察伊拉克黄芪总黄酮对细胞系和人肺成纤维细胞正常细胞系(WISH)的生物学影响。方法:采用反射仪提取植物粉末,薄层色谱法测定总黄酮含量。采用细胞毒试验(MTT)测定所制植物对人乳腺癌(MCF-7)、人宫颈癌(HELA)、人结肠癌(Caco-2)和人肺成纤维细胞正常细胞系(WISH)的细胞毒活性。结果:薄层色谱法检出芦丁、槲皮素、山奈酚、木犀草素等黄酮类化合物。与阴性对照相比,提取物对MCF-7和Caco-2的抑制作用在100µg/ml浓度下最高,分别为82.158%和61.360%,对Hela细胞株的抑制作用在100µg/ml浓度下最高,为54.880%。此外,总黄酮提取物浓度(6.25µg/ml)对正常WISH细胞株生长的抑制作用为(1.094%)。结论:木犀草中黄酮类化合物含量较高,采用薄层色谱法可检测出部分黄酮类化合物的定性。总黄酮在体外对不同类型的癌细胞和正常细胞株均表现出明显的细胞毒活性,其抗肿瘤活性呈剂量依赖性和细胞类型依赖性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In Vitro Cytotoxic Activity of Total Flavonoid from Equisetum Arvense Extract.

Background: Normally happening substances like flavonoids are regarded as active candidates for the treatment and prevention of cancer The purpose of this study was to see how Iraqi E. arvense total flavonoid affected cell lines biologically and human lung fibroblast normal cell line (WISH).

Methods: Plant powder was extracted by reflex apparatus, then thin-layer chromatography (TLC) was used to determine total flavonoids. Cytotoxicity assay (MTT) was used to determine the cytotoxic activity of the prepared plant against human breast cancer (MCF-7), cells human cervix cancer (HELA), human colon cancer (Caco-2) and human lung fibroblast normal cell line (WISH).

Results: The flavonoids Rutin, Quercetin, Kaempferol, and luteolin were detected using the Thin Layer Chromatography (TLC) technique. In contrast to the negative control, the extract inhibited cell growth to a highest of 82.158% for MCF-7 and 61.360% for Caco-2 at the concentration (100 µg/ml), and (54.880%) for Hela cell line at the concentration (100 µg/ml). In addition, the concentration (6.25 µg/ml) of total flavonoid extract produced a decrease in the growth of the normal WISH cell line to reach (1.094%).

Conclusion: Equisetum arvense contain high amounts of flavonoids, the qualification of some flavonoids compounds was detected using TLC. The total flavonoids showed significant cytotoxic activity against various types of cancer cell lines and normal cell line in vitro, the antitumor activity was highly efficient in a dose and cell type dependent manner.

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来源期刊
Reports of Biochemistry and Molecular Biology
Reports of Biochemistry and Molecular Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
2.80
自引率
23.50%
发文量
60
审稿时长
10 weeks
期刊介绍: The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.
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