能够降解三乙酸硝基的细菌的分离、特性和生理

T. Egli, H. Weilenmann
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引用次数: 4

摘要

直到最近,只有三种专需氧细菌(隶属于伪单胞菌属)可以以三乙酸硝基(NTA)作为其唯一的碳、氮和能量来源生长,在纯培养中被分离和研究。通过采用与以前不同的分离策略,在纯培养中从土壤和废水中分离出几种能够在好氧生长条件下利用NTA的非假单胞菌。此外,从河流沉积物中分离出一种在缺氧情况下能够利用NTA的反硝化细菌。这些分离物在细胞形态和生理方面具有特征。迄今收集的数据不能对分离的革兰氏阴性和革兰氏阳性菌株进行分类,而且分离株的分类位置仍然不清楚。然而,C1利用、乙酰素生产和不动性等特性清楚地表明,革兰氏阴性菌株不属于假单胞菌属。有关nta代谢酶在分离菌株TE 1中调控的信息表明,这些酶在这种细菌中是可诱导的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolation, characterization, and physiology of bacteria able to degrade nitrilotriacetate
Until recently, only three obligately aerobic bacteria (affiliated with the genus Pseudo-monas) that can grow with nitrilotriacetate (NTA) as their only source of carbon, nitrogen, and energy have been isolated and studied in pure culture. By employing a different isolation strategy than was used previously, several nonpseudomonads were isolated in pure culture from both soil and wastewater that are able to utilize NTA under aerobic growth conditions. Additionally, a denitrifying bacterium was isolated from river sediment that is able to utilize NTA in the absence of oxygen. These isolates have been characterized with respect to their cell morphology and physiology. The data collected so far do not allow classification of both the gram-negative and the gram-positive strains isolated, and the taxonomic position of the isolates remains obscure. However, properties like C1 utilization, production of acetoin, and nonmotility clearly indicate that the gram-negative strains do not belong to the genus Pseudomonas. Information is presented on the regulation of NTA-metabolizing enzymes in isolate TE 1 suggesting that these enzymes are inducible in this bacterium.
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