Kinetics of Petroleum Oil Recovery in Bio Surfactant of Brevundimonas diminuta and Pseudomonas citronellolis Bacteria Solutions

Kinetics of crude oil solubility in bio surfactant of Brevundimonas diminuta and Pseudomonas citronellolis bacterias was studied by using a combination of differential and integral methods. The order of reaction and reaction rate constants of crude oil solubility was determined by using differential and integral methods respectively. The observation was carried out for 10 days, and integral method observation was carried out every 2 days, for 10 days. The initial TPH concentration (Total Petroleum Hydrocarbon) of sludge sequently were 1.64; 3.28; 4.91; 6.11 and 7.57 % (w/w). After 10 days’ treatment by using B. diminuta bacteria, the TPH concentrations were decreased to 1.24; 2.43; 3.61; 4.32; 5.07% (w/w) and the TPH concentration using P. citronellolis decreased to 1.07; 2.10; 3.13; 3.79; 4.40% (w/w). The order reaction which determined by using differential method were 1.81 for B. diminuta and 1.1 for P. citronellolis. Furthermore, the order reaction was put in the integral equation thus forming reaction rate constants equation of crude oil solubility which are 0.018 day-1 for B. diminuta and 0.033 day-1 for P. citronellolis. Qualitative analysis which were carried out by using GC showed that bio surfactant from B. diminuta was able to dissolve hydrocarbon compounds of crude at fraction with lower C atomic chain C22. Bio surfactant from P. citronellolis was able to dissolve hydrocarbon compound with atomic chain C10 – C14.