Yanan Sun, Nan Li, Yuru Cai, Xingnan Zhao, Hongyu Yang
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引用次数: 0
摘要
核因子-κB (NF-κB)参与宫颈癌的发生发展。将Ca Ski细胞皮下接种于BALB/c裸鼠,构建异种移植模型。用六甲基槲皮素(HTQC)刺激或不刺激肿瘤坏死因子(TNF)α的宫颈癌细胞和异种移植小鼠的代表性肿瘤组织,检测NF-κB p65、p-p65、i - b α和p- i -κB α的相对蛋白表达。HTQC能抑制Ca - Ski和c - 33a细胞中NF-κB源性荧光素酶活性,抑制NF-κB p-p65和p- i -κB α的相对表达。同时,HTQC以浓度依赖的方式抑制体外细胞存活,抑制异种移植瘤模型的肿瘤体积和重量。综上所述,HTQC可作为NF-κB抑制剂抑制宫颈癌的生存和增殖,可作为未来临床治疗NF-κB的靶向药物。
The polymethoxylated flavone hexamethylquercetagetin suppresses NF-κB signaling and inhibits cell survival in cervical carcinoma.
Nuclear factor-κB (NF-κB) contributes to the development and progression of cervical carcinoma. To construct a xenograft model, Ca Ski cells were subcutaneously inoculated into BALB/c nude mice. The relative protein expression of NF-κB p65, p-p65, IκBα, and p-IκBα were detected in hexamethylquercetagetin (HTQC) treated cervical carcinoma cells with or without tumor necrosis factor (TNF)α stimulation, or representative tumors tissues in xenograft mice. HTQC could prohibit NF-κB-derived luciferase activity in Ca Ski and C-33 A cells and inhibit the relative NF-κB p-p65 and p-IκBα expression with or without TNFα stimulation. At the same time, HTQC inhibited in vitro cell survival in a concentration-dependent manner and suppressed the tumor volume and weight in xenograft models. In summary, HTQC functions as an NF-κB inhibitor to prohibit the survival and proliferation of cervical carcinoma, which can be considered as an NF-κB target remedy in future clinical practice.
期刊介绍:
Growth Factors is an international and interdisciplinary vehicle publishing new knowledge and findings on the regulators of cell proliferation, differentiation and survival. The Journal will publish research papers, short communications and reviews on current developments in cell biology, biochemistry, physiology or pharmacology of growth factors, cytokines or hormones which improve our understanding of biology or medicine. Among the various fields of study topics of particular interest include: •Stem cell biology •Growth factor physiology •Structure-activity relationships •Drug development studies •Clinical applications