单性亚马逊鲂(Poecilia formosa)的着丝粒减数分裂。

IF 2.4 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Chromosome Research Pub Date : 2022-12-01 Epub Date: 2022-12-02 DOI:10.1007/s10577-022-09708-2
Dmitrij Dedukh, Irene da Cruz, Susanne Kneitz, Anatolie Marta, Jenny Ormanns, Tomáš Tichopád, Yuan Lu, Manfred Alsheimer, Karel Janko, Manfred Schartl
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引用次数: 0

摘要

产生克隆后代的单性繁殖是有性繁殖的另一种策略,甚至在脊椎动物中也会出现。人们已经描述了多种非有性生殖模式,其中最不为人所知的一个问题是这种途径是如何出现的,以及它们是如何从机理上进行的。亚马逊鲂(Poecilia formosa)需要来自相关物种雄性的精子来触发二倍体卵子的孤雌生殖发育。然而,未还原的雌配子是如何产生的,其机制仍不清楚。细胞学分析表明,初级卵母细胞的染色体启动了pachytene,但没有进行二价形成和减数分裂交叉。比较 P. formosa 和其有性亲本的卵巢转录组发现,减数分裂特异性基因的表达水平与 P. mexicana 不同,但与 P. latipinna 不同。此外,一些减数分裂基因的表达偏向于亲本基因组的两个等位基因之一。我们从我们的数据中推断,在亚马逊小骡子中,二倍体卵母细胞是由于同源染色体的突触失败而通过无性繁殖产生的。这种失败并没有反映在已知减数分裂基因的差异表达上,这一事实表明其潜在的分子机制可能是蛋白质水平的失调或迄今未知的减数分裂基因的错误表达,以及/或由于来自不同基因组的蛋白质相互作用受损而导致的杂交育种不良。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Achiasmatic meiosis in the unisexual Amazon molly, Poecilia formosa.

Achiasmatic meiosis in the unisexual Amazon molly, Poecilia formosa.

Unisexual reproduction, which generates clonal offspring, is an alternative strategy to sexual breeding and occurs even in vertebrates. A wide range of non-sexual reproductive modes have been described, and one of the least understood questions is how such pathways emerged and how they mechanistically proceed. The Amazon molly, Poecilia formosa, needs sperm from males of related species to trigger the parthenogenetic development of diploid eggs. However, the mechanism, of how the unreduced female gametes are produced, remains unclear. Cytological analyses revealed that the chromosomes of primary oocytes initiate pachytene but do not proceed to bivalent formation and meiotic crossovers. Comparing ovary transcriptomes of P. formosa and its sexual parental species revealed expression levels of meiosis-specific genes deviating from P. mexicana but not from P. latipinna. Furthermore, several meiosis genes show biased expression towards one of the two alleles from the parental genomes. We infer from our data that in the Amazon molly diploid oocytes are generated by apomixis due to a failure in the synapsis of homologous chromosomes. The fact that this failure is not reflected in the differential expression of known meiosis genes suggests the underlying molecular mechanism may be dysregulation on the protein level or misexpression of a so far unknown meiosis gene, and/or hybrid dysgenesis because of compromised interaction of proteins from diverged genomes.

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来源期刊
Chromosome Research
Chromosome Research 生物-生化与分子生物学
CiteScore
4.70
自引率
3.80%
发文量
31
审稿时长
1 months
期刊介绍: Chromosome Research publishes manuscripts from work based on all organisms and encourages submissions in the following areas including, but not limited, to: · Chromosomes and their linkage to diseases; · Chromosome organization within the nucleus; · Chromatin biology (transcription, non-coding RNA, etc); · Chromosome structure, function and mechanics; · Chromosome and DNA repair; · Epigenetic chromosomal functions (centromeres, telomeres, replication, imprinting, dosage compensation, sex determination, chromosome remodeling); · Architectural/epigenomic organization of the genome; · Functional annotation of the genome; · Functional and comparative genomics in plants and animals; · Karyology studies that help resolve difficult taxonomic problems or that provide clues to fundamental mechanisms of genome and karyotype evolution in plants and animals; · Mitosis and Meiosis; · Cancer cytogenomics.
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