含有牛蒡素的牛蒡芽提取物通过调节小鼠肠道微生物群来预防肥胖。

IF 3.1 4区 医学 Q2 Agricultural and Biological Sciences
Shimpei Watanabe, Akiko Ohno, Satoshi Yomoda, Satoshi Inamasu
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引用次数: 0

摘要

几项研究表明,肠道微生物群会影响宿主的健康。例如,厚壁菌门/拟杆菌门(F/B)比例和嗜粘阿克曼氏菌在微生物群中的比例与肥胖密切相关。在这项研究中,我们评估了抗肥胖木脂素化合物牛蒡素(AG)和含有AG的牛蒡芽提取物(GSE)对肥胖小鼠模型肠道微生物群的影响。C57BL/6J小鼠分别饲喂含AG、GSE或二甲双胍的高脂高糖(HFHS)饲粮8周。采用16S rRNA基因测序和高效液相色谱法分别测定肠道菌群组成和盲肠短链脂肪酸含量。用AG、GSE和MF处理的小鼠体重增加明显受到抑制。肠道菌群分析显示,AG和gse处理组的F/B比显著降低。此外,AG和GSE处理的肥胖小鼠粪便中嗜粘杆菌的拷贝数显著增加。此外,AG和GSE处理后,盲肠内容物及其粪便中scfa(乙酸、丙酸和丁酸)的含量也显著增加。综上所述,这些结果表明,AG和GSE通过改善肠道微生物群的组成来预防肥胖。AG还能促进嗜粘杆菌的体外生长。因此,AG和GSE可能作为新型益生元补充剂来改善肥胖、便秘和肠道疾病。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Arctigenin-containing burdock sprout extract prevents obesity in association with modulation of the gut microbiota in mice.

Arctigenin-containing burdock sprout extract prevents obesity in association with modulation of the gut microbiota in mice.

Arctigenin-containing burdock sprout extract prevents obesity in association with modulation of the gut microbiota in mice.

Arctigenin-containing burdock sprout extract prevents obesity in association with modulation of the gut microbiota in mice.

Several studies have suggested that the gut microbiota affect the health of the host. For example, the Firmicutes/Bacteroidetes (F/B) ratio and the proportion of Akkermansia muciniphila in the microbiota have been closely linked to obesity. In this study, we evaluated the effects of an anti-obesity lignan compound, arctigenin (AG), and burdock sprout extract (GSE), which contains AG, on the gut microbiota of an obese mouse model. C57BL/6J mice were fed high-fat, high-sucrose (HFHS) diets containing AG, GSE, or metformin (MF) for 8 weeks. The composition of the gut microbiota and the cecal content of short-chain fatty acids (SCFAs) were determined using 16S rRNA gene sequencing and high-performance liquid chromatography, respectively. Body weight gain was significantly suppressed in mice treated with AG, GSE, and MF. Analysis of the gut microbiota revealed that the F/B ratio was significantly reduced in the AG- and GSE-treated groups. Furthermore, the copy number of A. muciniphila in the feces was significantly increased in obese mice treated with AG and GSE. In addition, the amount of SCFAs (acetic, propionic, and butyric acids) in the cecal content and their fecal excretions were also significantly increased following AG and GSE treatment. Taken together, these results suggest that AG and GSE prevent obesity by improving the composition of the gut microbiota. Moreover, AG promoted the growth of A. muciniphila in vitro. Thus, AG and GSE may function as novel prebiotic supplements to ameliorate obesity, constipation, and intestinal disorders.

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来源期刊
Bioscience of Microbiota, Food and Health
Bioscience of Microbiota, Food and Health Immunology and Microbiology-Applied Microbiology and Biotechnology
CiteScore
5.50
自引率
3.20%
发文量
24
期刊介绍: Bioscience of Microbiota, Food and Health (BMFH) is a peer-reviewed scientific journal with a specific area of focus: intestinal microbiota of human and animals, lactic acid bacteria (LAB) and food immunology and food function. BMFH contains Full papers, Notes, Reviews and Letters to the editor in all areas dealing with intestinal microbiota, LAB and food immunology and food function. BMFH takes a multidisciplinary approach and focuses on a broad spectrum of issues.
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