Fatemeh Fathi, Ali Zarei Mahmoudabadi, Mahnaz Fatahinia
{"title":"从HIV+/AIDS患者和健康人分离的白色念珠菌和非白色念珠菌胞外水解酶的比较研究","authors":"Fatemeh Fathi, Ali Zarei Mahmoudabadi, Mahnaz Fatahinia","doi":"10.18502/cmm.8.2.10330","DOIUrl":null,"url":null,"abstract":"<p><strong>Background and purpose: </strong>Oropharyngeal candidiasis is the most prevalent opportunistic fungal infection in patients with human immunodeficiency virus (HIV) as well as other immunodeficiency disorders, which is caused by various <i>Candida</i> species, mostly <i>Candida albicans</i>. Studies have shown that <i>Candida</i> isolates differ in their pathogenicity. These variations are attributed to virulence factors, host characteristics, and the target tissue. This study aimed to determine and compare the secretion of hydrolytic enzymes in <i>C. albicans</i> and non-<i>albicans Candida</i> species isolated from HIV<sup>+</sup>/AIDS patients and healthy individuals.</p><p><strong>Materials and methods: </strong>Samples were taken from 201 patients with HIV and 118 healthy individuals. The samples were identified by macroscopic, phenotypic, and molecular methods, and virulence factors were subsequently measured. Statistical differences in enzymatic activity of various <i>Candida</i> isolates were calculated (<i>P</i><0.0001).</p><p><strong>Results: </strong>In total, 95 samples (47.20%) from patients and 46 samples (38.90%) from healthy individuals were positive for the growth of different Candida species. There were 39 (41.10%) and 36 (78.30%) <i>C. albicans</i> in patients and healthy individuals, respectively, as well as 56 (58.90%) and 10 (21.70%) non-<i>albicans</i> species in patients and healthy subjects, respectively. All the enzymes produced by <i>Candida</i> species enzymes were at low, medium, and high levels. Hemolysin activity in <i>Candida</i> species isolated from patients was significantly higher, compared to healthy individuals. Moreover, the activity of all <i>C. albicans</i> enzymes in patients was significantly higher than other <i>Candida</i> species.</p><p><strong>Conclusion: </strong>The <i>C. albicans</i> isolated from HIV-positive individuals secreted higher amounts of exoenzymes, and can cause oropharyngeal candidiasis and become a source of candidiasis for the host.</p>","PeriodicalId":10863,"journal":{"name":"Current Medical Mycology","volume":"8 2","pages":"32-39"},"PeriodicalIF":0.0000,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9825789/pdf/","citationCount":"0","resultStr":"{\"title\":\"A comparative study on the production of extracellular hydrolytic enzymes of <i>C. albicans</i> and non-<i>albicans Candida</i> species isolated from HIV<sup>+</sup>/AIDS patients and healthy individuals.\",\"authors\":\"Fatemeh Fathi, Ali Zarei Mahmoudabadi, Mahnaz Fatahinia\",\"doi\":\"10.18502/cmm.8.2.10330\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background and purpose: </strong>Oropharyngeal candidiasis is the most prevalent opportunistic fungal infection in patients with human immunodeficiency virus (HIV) as well as other immunodeficiency disorders, which is caused by various <i>Candida</i> species, mostly <i>Candida albicans</i>. Studies have shown that <i>Candida</i> isolates differ in their pathogenicity. These variations are attributed to virulence factors, host characteristics, and the target tissue. This study aimed to determine and compare the secretion of hydrolytic enzymes in <i>C. albicans</i> and non-<i>albicans Candida</i> species isolated from HIV<sup>+</sup>/AIDS patients and healthy individuals.</p><p><strong>Materials and methods: </strong>Samples were taken from 201 patients with HIV and 118 healthy individuals. The samples were identified by macroscopic, phenotypic, and molecular methods, and virulence factors were subsequently measured. Statistical differences in enzymatic activity of various <i>Candida</i> isolates were calculated (<i>P</i><0.0001).</p><p><strong>Results: </strong>In total, 95 samples (47.20%) from patients and 46 samples (38.90%) from healthy individuals were positive for the growth of different Candida species. There were 39 (41.10%) and 36 (78.30%) <i>C. albicans</i> in patients and healthy individuals, respectively, as well as 56 (58.90%) and 10 (21.70%) non-<i>albicans</i> species in patients and healthy subjects, respectively. All the enzymes produced by <i>Candida</i> species enzymes were at low, medium, and high levels. Hemolysin activity in <i>Candida</i> species isolated from patients was significantly higher, compared to healthy individuals. Moreover, the activity of all <i>C. albicans</i> enzymes in patients was significantly higher than other <i>Candida</i> species.</p><p><strong>Conclusion: </strong>The <i>C. albicans</i> isolated from HIV-positive individuals secreted higher amounts of exoenzymes, and can cause oropharyngeal candidiasis and become a source of candidiasis for the host.</p>\",\"PeriodicalId\":10863,\"journal\":{\"name\":\"Current Medical Mycology\",\"volume\":\"8 2\",\"pages\":\"32-39\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9825789/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Current Medical Mycology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18502/cmm.8.2.10330\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Medical Mycology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/cmm.8.2.10330","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
A comparative study on the production of extracellular hydrolytic enzymes of C. albicans and non-albicans Candida species isolated from HIV+/AIDS patients and healthy individuals.
Background and purpose: Oropharyngeal candidiasis is the most prevalent opportunistic fungal infection in patients with human immunodeficiency virus (HIV) as well as other immunodeficiency disorders, which is caused by various Candida species, mostly Candida albicans. Studies have shown that Candida isolates differ in their pathogenicity. These variations are attributed to virulence factors, host characteristics, and the target tissue. This study aimed to determine and compare the secretion of hydrolytic enzymes in C. albicans and non-albicans Candida species isolated from HIV+/AIDS patients and healthy individuals.
Materials and methods: Samples were taken from 201 patients with HIV and 118 healthy individuals. The samples were identified by macroscopic, phenotypic, and molecular methods, and virulence factors were subsequently measured. Statistical differences in enzymatic activity of various Candida isolates were calculated (P<0.0001).
Results: In total, 95 samples (47.20%) from patients and 46 samples (38.90%) from healthy individuals were positive for the growth of different Candida species. There were 39 (41.10%) and 36 (78.30%) C. albicans in patients and healthy individuals, respectively, as well as 56 (58.90%) and 10 (21.70%) non-albicans species in patients and healthy subjects, respectively. All the enzymes produced by Candida species enzymes were at low, medium, and high levels. Hemolysin activity in Candida species isolated from patients was significantly higher, compared to healthy individuals. Moreover, the activity of all C. albicans enzymes in patients was significantly higher than other Candida species.
Conclusion: The C. albicans isolated from HIV-positive individuals secreted higher amounts of exoenzymes, and can cause oropharyngeal candidiasis and become a source of candidiasis for the host.