赭曲霉次生代谢产物研究 Study on the Secondary Metabolites of Aspergillus ochraceus

目的：研究赭曲霉次生代谢产物。方法：利用多种色谱层析方法分离出次生代谢产物，通过ESI-MS/MS及NMR等波谱方法鉴定结构。结果：共分离鉴定出9种化合物，依次是赭曲霉毒素A(1)、赭曲霉毒素B(2)、neohydroxyaspergillic acid(3)、(3R)-5-hydroxymellein(4)、5,6-dihydropenicillic acid(5)、mesaconic acid(6)、p-hydroxybenzoic acid(7)、circumdatin G(8)、(22E,24R)-ergosta-7,22-diene-3β,5α,6β- triol(9)。结论：化合物3~9为首次从赭曲霉中分离得到的化合物。 Objective: To study the secondary metabolites from Aspergillus ochraceus. Methods: The constit-uents were isolated and purified by column chromatography and preparative TLC. Their structures were identified on the basis of comprehensive spectroscopic methods including ESI-MS and spectral data (1H, 13C-NMR). Results: Nine compounds were isolated and identified as ochratoxin A (1), ochratoxin B (2), neohydroxyaspergillic acid (3), (3R)-5-hydroxymellein (4), 5,6-dihydro- penicillic acid (5), mesaconic acid (6), p-hydroxybenzoic acid (7), circumdatin G (8), (22E,24R)- ergosta-7,22-diene-3β,5α,6β-triol (9). Conclusion: The compounds 3~9 were isolated from Asper-gillus ochraceus for the first time.