可卡因使用障碍和童年虐待与中性粒细胞活化和炎症加剧有关。

IF 3.8 4区 医学 Q1 Medicine
Acta Neuropsychiatrica Pub Date : 2024-04-01 Epub Date: 2023-02-27 DOI:10.1017/neu.2023.11
Giselle A Funchal, Jaqueline B Schuch, Aline Zaparte, Breno Sanvicente-Vieira, Thiago W Viola, Rodrigo Grassi-Oliveira, Moisés E Bauer
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引用次数: 0

摘要

背景:可卡因使用障碍(CUD)与早期生活逆境和激活的细胞免疫反应有关。女性最容易受到慢性药物紊乱并发症的影响,她们通常表现出强烈的戒断感,并消耗大量的药物。在这里,我们研究了中性粒细胞在 CUD 中的功能活动,包括中性粒细胞胞外陷阱(NET)的形成和相关的胞内信号传导。我们还研究了早期生活压力在炎症特征中的作用:方法:我们在 41 名女性 CUD 患者和 31 名健康对照者(HCs)开始戒毒治疗时收集了他们的血样、临床数据和童年虐待或忽视史。通过流式细胞术评估血浆细胞因子、中性粒细胞吞噬能力、NETs、细胞内活性氧(ROS)生成、磷酸化蛋白激酶B(Akt)和丝裂原活化蛋白激酶(MAPK):结果:与对照组相比,CUD 受试者的童年创伤评分更高。与对照组相比,CUD受试者的血浆细胞因子(TNF-α、IL-1β、IL-6、IL-8、IL-12和IL-10)、中性粒细胞吞噬能力和NET的产生均有所增加。CUD 受试者的中性粒细胞还产生高水平的细胞内 ROS,并有更多的 Akt 和 MAPKs(p38/ERK)活化,这些都是参与细胞存活和 NETs 生成的重要信号通路。童年创伤评分与中性粒细胞活化和外周炎症显著相关:我们的研究证实,熏可卡因和早期生活压力会在炎症环境中激活中性粒细胞。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cocaine-use disorder and childhood maltreatment are associated with the activation of neutrophils and increased inflammation.

Background: Cocaine-use disorder (CUD) has been associated with early life adversity and activated cellular immune responses. Women are most vulnerable to complications from chronic substance disorders, generally presenting an intense feeling of abstinence and consuming significant drug amounts. Here, we investigated neutrophil functional activities in CUD, including the formation of neutrophil extracellular traps (NETs) and related intracellular signalling. We also investigated the role of early life stress in inflammatory profiles.

Methods: Blood samples, clinical data, and history of childhood abuse or neglect were collected at the onset of detoxification treatment of 41 female individuals with CUD and 31 healthy controls (HCs). Plasma cytokines, neutrophil phagocytosis, NETs, intracellular reactive oxygen species (ROS) generation, and phosphorylated protein kinase B (Akt) and mitogen-activated protein kinases (MAPK)s were assessed by flow cytometry.

Results: CUD subjects had higher scores of childhood trauma than controls. Increased plasma cytokines (TNF-α, IL-1β, IL-6, IL-8, IL-12, and IL-10), neutrophil phagocytosis, and production of NETs were reported in CUD subjects as compared to HC. Neutrophils of CUD subjects also produced high levels of intracellular ROS and had more activated Akt and MAPKs (p38/ERK), which are essential signalling pathways involved in cell survival and NETs production. Childhood trauma scores were significantly associated with neutrophil activation and peripheral inflammation.

Conclusion: Our study reinforces that smoked cocaine and early life stress activate neutrophils in an inflammatory environment.

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来源期刊
Acta Neuropsychiatrica
Acta Neuropsychiatrica 医学-精神病学
CiteScore
8.50
自引率
5.30%
发文量
30
审稿时长
6-12 weeks
期刊介绍: Acta Neuropsychiatrica is an international journal focussing on translational neuropsychiatry. It publishes high-quality original research papers and reviews. The Journal''s scope specifically highlights the pathway from discovery to clinical applications, healthcare and global health that can be viewed broadly as the spectrum of work that marks the pathway from discovery to global health.
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