J.A Cabezas, J.V Porto, M.D Frois, C Marino, J Arzúa
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引用次数: 27
摘要
n -乙酰神经氨酸已在人的眼泪中被鉴定出来;其他伴随的唾液酸尚未被发现。n -乙酰神经氨酸不是游离的，而是发现的。用0.1 N HCl或0.1 N H2SO4在80°条件下，用神经氨酸酶(唾液酸酶)(N-乙酰神经氨酸糖水解酶，EC 3.2.1.18)在pH 5.5和37°条件下水解约45分钟，可明显释放α-血清红蛋白。纸电泳显示其主要分布在色谱图的一部分，迁移率为−3.8·10−5 cm2 sec−1 V−1，与α-血清红蛋白相对应。测定了泪液中己糖和己糖胺的平均浓度，并讨论了其生化意义。
N-Acetylneuraminic acid has been identified in human tears; other accompanying sialic acids have not been found. N-Acetylneuraminic acid has not been found free, but found. A remarkable release is easily produced by hydrolysis with 0.1 N HCl or 0.1 N H2SO4 at 80° and with neuraminidase (sialidase) (N-acetylneuraminate glycohydrolase, EC 3.2.1.18) at pH 5.5 and 37°, after about 45 min.
Paper electrophoresis shows its main distribution in a fraction of the pherogram with a mobility of −3.8·10−5 cm2 sec−1 V−1 which would correspond to the α-seroglobulins.
The mean concentration in tears is determined, as well as that of hexoses and hexosamines, and a discussion is made about its biochemical signification.
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