B. Sen-Utsukarci, N. Tabanca, A. Estep, Ozlem Akbal-Dagistan, S. Kessler, Z. Ozturk, J. Becnel, A. Kiemer, A. Mat
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引用次数: 0
摘要
飞燕草是众所周知的有毒植物,含有二萜生物碱。对台湾飞燕草的细胞毒性研究尚未见报道。和休伊特。研究了土耳其特有植物D. formosum不同部位提取物对人肝癌细胞株HepG2和人脐静脉内皮细胞(HUVEC)的细胞毒活性。采用MTT法测定了12种提取物和亚组分对HepG2细胞的细胞毒作用。唯一的活性提取物应用于HUVEC作为健康细胞的模型。对HepG2和HUVEC的IC50分别为244,9 μg/mL和144,4 μg/mL,根部提取物对HepG2和HUVEC均无活性。结果是不可能的,尽管它预测了次生代谢产物,如二萜生物碱(甲基茄头碱,棕色碱,番茄碱,阿瓦达定,antranoyllycoctonine, delsemine A/B和番茄碱)。根据以往的文献研究,细胞毒性植物也有望表现出杀虫活性。为此,我们检测了台湾金丝梅细胞毒提取物对黄热病、登革热和寨卡病毒载体埃及伊蚊的杀虫活性。本文可引为:Sen-Utsukarci B, Tabanca N, Estep as, Akbal-Dagistan O, Kessler SM, Ozturk Z, Becnel J, Kiemer AK, Mat A(2019)。台湾飞燕草提取物的细胞毒性和杀虫活性。和休伊特。IstanbulJPharm 10.26650/ istanbuljpharma .2019.19034。
The Cytotoxicity and Insecticidal Activity of Extracts from Delphinium formosum Boiss. & Huet
DOI : 10.26650/IstanbulJPharm.2019.19034 Delphinium species are well-known toxic plants with their diterpenoid alkaloid contents. There has been no previous investigation on the cytotoxicity of Delphinium formosum Boiss. & Huet. The extracts of the different parts of D. formosum, an endemic species in Turkey, were investigated for their cytotoxic activity against the human liver carcinoma cell line (HepG2) and primary human umbilical vein endothelial cells (HUVEC). The cytotoxic effects of twelve extracts and subfractions were determined against HepG2 cells using MTT assay. The only active extract was applied to the HUVEC as a model for healthy cells. Only one of the alkaloid-containing extracts from the aerial parts was toxic (IC50 = 244,9 μg/mL against HepG2 and 144,4 μg/mL against HUVEC), while the root extracts were inactive. The results were improbable although it’s predicted secondary metabolites, such as diterpene alkaloids (methyllycaconitine, browniine, lycoctonine, avardharidine, antranoyllycoctonine, delsemine A/B and lycoctonine). Based on previous studies in literature, the cytotoxic plants were also expected to exhibit insecticidal activity. Therefore, the cytotoxic extract of D. formosum was examined for its adulticidal and larvicidal activity against the yellow fever, dengue fevers and Zika virus vector Aedes aegypti L. You may cite this article as : Sen-Utsukarci B, Tabanca N, Estep AS, Akbal-Dagistan O, Kessler SM, Ozturk Z, Becnel J, Kiemer AK, Mat A (2019). The Cytotoxicity and Insecticidal Activity of Extracts from Delphinium formosum Boiss. & Huet. Istanbul J Pharm 10.26650/IstanbulJPharm.2019.19034.