哺乳动物核斑与染色质稳定相关:一项生化研究

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
K. Raina, B. Rao
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引用次数: 3

摘要

核斑点(Nuclear Speckles, NS)是蛋白质和RNA相分离凝聚体,其组分动态协调RNA转录、剪接、运输和DNA修复。NS主要通过成像研究来探索,历史上一直被称为染色质间颗粒团簇,其生化特性,特别是与染色质的关系在很大程度上未被探索。在这项研究中,我们测试了NS是否表现出与染色质的稳定联系,并表明染色质的有限dna -1切割导致NS崩溃成各向同性分布或组成蛋白的聚集,而不影响其他核结构。进一步的生化探测表明,NS蛋白与染色质紧密相关,与组蛋白一样,只能通过高盐处理提取。在mase消化染色质后,NS也与溶解的单二核体染色质部分共同释放。我们提出了一个模型,即NS-染色质构成了一个“假定的稳定关联”,其耦合可能受到染色质和NS变化的联合调节。缩写:NS:核斑;DSB:双链断裂;PTM:翻译后修饰;DDR: DNA损伤修复;RBP-RNA结合蛋白;TAD:拓扑关联域;LCR:低复杂度区域;IDR:内在无序区域。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mammalian nuclear speckles exhibit stable association with chromatin: a biochemical study
ABSTRACT Nuclear Speckles (NS) are phase-separated condensates of protein and RNA whose components dynamically coordinate RNA transcription, splicing, transport and DNA repair. NS, probed largely by imaging studies, remained historically well known as Interchromatin Granule Clusters, and biochemical properties, especially their association with Chromatin have been largely unexplored. In this study, we tested whether NS exhibit any stable association with chromatin and show that limited DNAse-1 nicking of chromatin leads to the collapse of NS into isotropic distribution or aggregates of constituent proteins without affecting other nuclear structures. Further biochemical probing revealed that NS proteins were tightly associated with chromatin, extractable only by high-salt treatment just like histone proteins. NS were also co-released with solubilised mono-dinucleosomal chromatin fraction following the MNase digestion of chromatin. We propose a model that NS-chromatin constitutes a “putative stable association” whose coupling might be subject to the combined regulation from both chromatin and NS changes. Abbreviations: NS: Nuclear speckles; DSB: double strand breaks; PTM: posttranslational modifications; DDR: DNA damage repair; RBP-RNA binding proteins; TAD: topologically associated domains; LCR: low complexity regions; IDR: intrinsically disordered regions.
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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