hplc - spme - GC-MS联合表征8种凤凰丹参茶和鲜叶挥发性成分

Jingfang Shi, Wenjie Huang, Zhuang Chen, Shili Sun, Limin Xiang, Q. Kong, Xiaohui Jiang, Dong Chen, Shijuan Yan
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引用次数: 8

摘要

凤凰丹琮茶以其优良的香气品质而闻名。为了研究凤凰单从茶不同香气类型的挥发性成分,采用顶空固相微萃取(HS-SPME) -气相色谱-质谱联用(GC-MS)技术,对同一产地、同一工艺生产的7种著名香气类型的鲜叶和成品茶及其祖先品种进行了研究。结果表明,黄枝、杏仁、桂花、米兰、江花、玉兰叶来、凤凰水仙等茶品与鲜叶的挥发性成分及含量存在明显差异。鲜叶挥发性成分主要为芳樟醇、(E)-2-己烯醛、醋酸顺-3-己烯酯、氧化芳樟醇、水杨酸甲酯、香叶醇和神经樟醇,总相对含量为78.44 ~ 90.07%。而在成品茶中,三烯醇、芳樟醇、β-月桂烯、d -柠檬烯、1-乙基- 1h -吡咯-2-甲醛、β-辛烯、氧化芳樟醇、苄基腈、吲哚、茉莉酮和橙花醇是主要挥发性成分,挥发性含量在60.12 ~ 93.97%之间。虽然不同香气类型的成品茶在香气成分和含量上有一定的相似性,但每一种香气类型都有其独特的香气特征。凤凰水仙与其他成品茶香气类型的明显差异可能是由于烯烃和吡咯衍生物的含量较高,而醇类,特别是萜烯醇的含量较低。此外,成品茶与鲜叶的相关性表明,鲜叶的挥发性化合物特征可能会影响成品茶的香气品质。本研究对不同香气类型的凤凰丹丛茶的挥发性成分进行了全面比较,为今后进一步评价凤凰丹丛茶的品质提供了科学依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characterization of Volatile Components of Eight FengHuang Dancong Manufactured Teas and Fresh Leaves by HS-SPME Coupled with GC-MS
FengHuang Dancong tea is famous for its excellent aroma quality. In order to characterize the volatile components in different aroma types of FengHuang Dancong tea, both fresh leaves and manufactured teas of seven well-known aroma types and their ancestor variety, which were harvested from the same places and manufactured using the same procedure, were investigated using headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). Results indicated that the volatile composition and contents of manufactured teas and fresh leaves, including HuangZhi, XingRen, GuiHua, MiLan, JiangHua, YuLan YeLai and Fenghuang Shuixian, were obviously different. Linalool, (E)-2-hexenal, cis-3-hexenyl acetate, linalool oxide, methyl salicylate, geraniol, and nerolidol were the major volatile components in fresh leaves, and their total relative contents ranged from 78.44-90.07%. But in manufactured teas, hotrienol, linalool, β-myrcene, D-limonene, 1-ethyl-1H-pyrrole-2-carboxaldehyde, β-ocimene, linalool oxide, benzyl nitrile, indole, jasmone, and nerolidol were the major volatile components, ranged from 60.12-93.97%. Although there were some similarities in the aroma composition and content among the manufactured teas of different aroma types, each type had unique aroma characteristics. The obvious difference between FengHuang Shuixian and other aroma types of manufactured teas may be due to the higher content of alkene and pyrrole derivatives and lower content of alcohols, especial terpene alcohols. Furthermore, the correlations between manufactured teas and the fresh leaves indicated that the volatile compounds profile of fresh leaves may affect the aroma quality of the manufactured tea. This study provided a comprehensive comparison of the volatile profile in different aroma types of Fenghuang Dancong tea, which is a scientific foundation for further quality assessment of Fenghuang Dancong variety in the future.
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