用于实时监测植物体内多种胁迫信号分子的体内表面增强拉曼散射纳米传感器

IF 38.1 1区 材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY
Won Ki Son, Yun Sik Choi, Young Woo Han, Dong Wook Shin, Kyunghun Min, Jiyoung Shin, Min Jeong Lee, Hokyoung Son, Dae Hong Jeong, Seon-Yeong Kwak
{"title":"用于实时监测植物体内多种胁迫信号分子的体内表面增强拉曼散射纳米传感器","authors":"Won Ki Son, Yun Sik Choi, Young Woo Han, Dong Wook Shin, Kyunghun Min, Jiyoung Shin, Min Jeong Lee, Hokyoung Son, Dae Hong Jeong, Seon-Yeong Kwak","doi":"10.1038/s41565-022-01274-2","DOIUrl":null,"url":null,"abstract":"When under stress, plants release molecules to activate their defense system. Detecting these stress-related molecules offers the possibility to address stress conditions and prevent the development of diseases. However, detecting endogenous signalling molecules in living plants remains challenging due to low concentrations of these analytes and interference with other compounds; additionally, many methods currently used are invasive and labour-intensive. Here we show a non-destructive surface-enhanced Raman scattering (SERS)-based nanoprobe for the real-time detection of multiple stress-related endogenous molecules in living plants. The nanoprobe, which is placed in the intercellular space, is optically active in the near-infrared region (785 nm) to avoid interferences from plant autofluorescence. It consists of a Si nanosphere surrounded by a corrugated Ag shell modified by a water-soluble cationic polymer poly(diallyldimethylammonium chloride), which can interact with multiple plant signalling molecules. We measure a SERS enhancement factor of 2.9 × 107 and a signal-to-noise ratio of up to 64 with an acquisition time of ~100 ms. To show quantitative multiplex detection, we adopted a binding model to interpret the SERS intensities of two different analytes bound to the SERS hot spot of the nanoprobe. Under either abiotic or biotic stress, our optical nanosensors can successfully monitor salicylic acid, extracellular adenosine triphosphate, cruciferous phytoalexin and glutathione in Nasturtium officinale, Triticum aestivum L. and Hordeum vulgare L.—all stress-related molecules indicating the possible onset of a plant disease. We believe that plasmonic nanosensor platforms can enable the early diagnosis of stress, contributing to a timely disease management of plants. A non-destructive surface-enhanced-Raman-scattering-based nanoprobe detects multiple endogenous molecules in living plants that are released under either abiotic or biotic stress, indicating the possible onset of a disease.","PeriodicalId":18915,"journal":{"name":"Nature nanotechnology","volume":"18 2","pages":"205-216"},"PeriodicalIF":38.1000,"publicationDate":"2022-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"14","resultStr":"{\"title\":\"In vivo surface-enhanced Raman scattering nanosensor for the real-time monitoring of multiple stress signalling molecules in plants\",\"authors\":\"Won Ki Son, Yun Sik Choi, Young Woo Han, Dong Wook Shin, Kyunghun Min, Jiyoung Shin, Min Jeong Lee, Hokyoung Son, Dae Hong Jeong, Seon-Yeong Kwak\",\"doi\":\"10.1038/s41565-022-01274-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"When under stress, plants release molecules to activate their defense system. Detecting these stress-related molecules offers the possibility to address stress conditions and prevent the development of diseases. However, detecting endogenous signalling molecules in living plants remains challenging due to low concentrations of these analytes and interference with other compounds; additionally, many methods currently used are invasive and labour-intensive. Here we show a non-destructive surface-enhanced Raman scattering (SERS)-based nanoprobe for the real-time detection of multiple stress-related endogenous molecules in living plants. The nanoprobe, which is placed in the intercellular space, is optically active in the near-infrared region (785 nm) to avoid interferences from plant autofluorescence. It consists of a Si nanosphere surrounded by a corrugated Ag shell modified by a water-soluble cationic polymer poly(diallyldimethylammonium chloride), which can interact with multiple plant signalling molecules. We measure a SERS enhancement factor of 2.9 × 107 and a signal-to-noise ratio of up to 64 with an acquisition time of ~100 ms. To show quantitative multiplex detection, we adopted a binding model to interpret the SERS intensities of two different analytes bound to the SERS hot spot of the nanoprobe. Under either abiotic or biotic stress, our optical nanosensors can successfully monitor salicylic acid, extracellular adenosine triphosphate, cruciferous phytoalexin and glutathione in Nasturtium officinale, Triticum aestivum L. and Hordeum vulgare L.—all stress-related molecules indicating the possible onset of a plant disease. We believe that plasmonic nanosensor platforms can enable the early diagnosis of stress, contributing to a timely disease management of plants. A non-destructive surface-enhanced-Raman-scattering-based nanoprobe detects multiple endogenous molecules in living plants that are released under either abiotic or biotic stress, indicating the possible onset of a disease.\",\"PeriodicalId\":18915,\"journal\":{\"name\":\"Nature nanotechnology\",\"volume\":\"18 2\",\"pages\":\"205-216\"},\"PeriodicalIF\":38.1000,\"publicationDate\":\"2022-12-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"14\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nature nanotechnology\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://www.nature.com/articles/s41565-022-01274-2\",\"RegionNum\":1,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MATERIALS SCIENCE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nature nanotechnology","FirstCategoryId":"88","ListUrlMain":"https://www.nature.com/articles/s41565-022-01274-2","RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 14

摘要

植物在受到胁迫时会释放分子来激活防御系统。检测这些与胁迫相关的分子为应对胁迫条件和预防疾病的发生提供了可能。然而,在活体植物中检测内源信号分子仍然具有挑战性,因为这些分析物的浓度很低,而且会受到其他化合物的干扰;此外,目前使用的许多方法都是侵入性和劳动密集型的。在这里,我们展示了一种基于表面增强拉曼散射(SERS)的非破坏性纳米探针,用于实时检测活体植物中多种与胁迫相关的内源分子。该纳米探针置于细胞间隙中,在近红外区域(785 纳米)具有光学活性,以避免植物自发荧光的干扰。它由一个硅纳米球组成,纳米球周围是波纹状的银外壳,银外壳由水溶性阳离子聚合物聚(二烯丙基二甲基氯化铵)修饰,可与多种植物信号分子相互作用。我们测量到的 SERS 增强因子为 2.9 × 107,信噪比高达 64,采集时间约为 100 毫秒。为了显示定量多重检测,我们采用了一种结合模型来解释与纳米探针 SERS 热点结合的两种不同分析物的 SERS 强度。在非生物或生物胁迫条件下,我们的光学纳米传感器都能成功监测到金莲花、小麦和大麦中的水杨酸、细胞外三磷酸腺苷、十字花科植物毒素和谷胱甘肽--所有这些与胁迫相关的分子都预示着植物病害的可能发生。我们相信,质子纳米传感器平台可以实现对胁迫的早期诊断,从而有助于及时防治植物病害。基于非破坏性表面增强拉曼散射的纳米探针可检测活体植物中的多种内源分子,这些分子在非生物或生物胁迫下释放,预示着疾病的可能发生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

In vivo surface-enhanced Raman scattering nanosensor for the real-time monitoring of multiple stress signalling molecules in plants

In vivo surface-enhanced Raman scattering nanosensor for the real-time monitoring of multiple stress signalling molecules in plants
When under stress, plants release molecules to activate their defense system. Detecting these stress-related molecules offers the possibility to address stress conditions and prevent the development of diseases. However, detecting endogenous signalling molecules in living plants remains challenging due to low concentrations of these analytes and interference with other compounds; additionally, many methods currently used are invasive and labour-intensive. Here we show a non-destructive surface-enhanced Raman scattering (SERS)-based nanoprobe for the real-time detection of multiple stress-related endogenous molecules in living plants. The nanoprobe, which is placed in the intercellular space, is optically active in the near-infrared region (785 nm) to avoid interferences from plant autofluorescence. It consists of a Si nanosphere surrounded by a corrugated Ag shell modified by a water-soluble cationic polymer poly(diallyldimethylammonium chloride), which can interact with multiple plant signalling molecules. We measure a SERS enhancement factor of 2.9 × 107 and a signal-to-noise ratio of up to 64 with an acquisition time of ~100 ms. To show quantitative multiplex detection, we adopted a binding model to interpret the SERS intensities of two different analytes bound to the SERS hot spot of the nanoprobe. Under either abiotic or biotic stress, our optical nanosensors can successfully monitor salicylic acid, extracellular adenosine triphosphate, cruciferous phytoalexin and glutathione in Nasturtium officinale, Triticum aestivum L. and Hordeum vulgare L.—all stress-related molecules indicating the possible onset of a plant disease. We believe that plasmonic nanosensor platforms can enable the early diagnosis of stress, contributing to a timely disease management of plants. A non-destructive surface-enhanced-Raman-scattering-based nanoprobe detects multiple endogenous molecules in living plants that are released under either abiotic or biotic stress, indicating the possible onset of a disease.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Nature nanotechnology
Nature nanotechnology 工程技术-材料科学:综合
CiteScore
59.70
自引率
0.80%
发文量
196
审稿时长
4-8 weeks
期刊介绍: Nature Nanotechnology is a prestigious journal that publishes high-quality papers in various areas of nanoscience and nanotechnology. The journal focuses on the design, characterization, and production of structures, devices, and systems that manipulate and control materials at atomic, molecular, and macromolecular scales. It encompasses both bottom-up and top-down approaches, as well as their combinations. Furthermore, Nature Nanotechnology fosters the exchange of ideas among researchers from diverse disciplines such as chemistry, physics, material science, biomedical research, engineering, and more. It promotes collaboration at the forefront of this multidisciplinary field. The journal covers a wide range of topics, from fundamental research in physics, chemistry, and biology, including computational work and simulations, to the development of innovative devices and technologies for various industrial sectors such as information technology, medicine, manufacturing, high-performance materials, energy, and environmental technologies. It includes coverage of organic, inorganic, and hybrid materials.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信