Tatjana N. Zhilina , Dimitry Y. Sorokin , Stepan V. Toshchakov , Ilya V. Kublanov , Daria G. Zavarzina
{"title":"来自高盐碱湖的嗜盐嗜碱有机营养拟杆菌属Natronogracilivirga saccharolytica gen. nov.， sp. 11 .和Cyclonatronum proteinivorum gen. nov.， sp. 11 .，形成了一个新的Cyclonatronaceae家族。11月，balneolale目","authors":"Tatjana N. Zhilina , Dimitry Y. Sorokin , Stepan V. Toshchakov , Ilya V. Kublanov , Daria G. Zavarzina","doi":"10.1016/j.syapm.2023.126403","DOIUrl":null,"url":null,"abstract":"<div><p><span><span>Two heterotrophic bacteroidetes strains were isolated as satellites from autotrophic enrichments inoculated with samples from hypersaline soda lakes in southwestern </span>Siberia. Strain Z-1702</span><sup>T</sup><span> is an obligate anaerobic fermentative saccharolytic bacterium from an iron-reducing enrichment culture, while </span><em>Ca</em>. Cyclonatronum proteinivorum Omega<sup>T</sup><span> is an obligate aerobic proteolytic microorganism<span> from a cyanobacterial enrichment. Cells of isolated bacteria are characterized by highly variable morphology. Both strains are chloride-independent moderate salt-tolerant obligate alkaliphiles<span> and mesophiles. Strain Z-1702</span></span></span><sup>T</sup><span><span><span> ferments glucose, maltose, fructose, </span>mannose, </span>sorbose<span>, galactose<span>, cellobiose, </span></span></span><em>N</em><span><span>-acetyl-glucosamine and alpha-glucans, including starch, glycogen, dextrin, and </span>pullulan. Strain Omega</span><sup>T</sup><span> is strictly proteolytic utilizing a range of proteins and peptones<span>. The main polar lipid fatty acid in both strains is iso-C</span></span><sub>15:0</sub>, while other major components are various C<sub>16</sub> and C<sub>17</sub><span> isomers. According to pairwise sequence alignments using BLAST </span><em>Gracilimonas</em><span> was the nearest cultured relative to both strains (<90% of 16S rRNA gene sequence identity). Phylogenetic analysis placed strain Z-1702</span><sup>T</sup> and strain Omega<sup>T</sup> as two different genera in a deep-branching clade of the new family level within the order <em>Balneolales</em> with genus. Based on physiological characteristics and phylogenetic position of strain Z-1702<sup>T</sup> it was proposed to represent a novel genus and species <em>Natronogracilivirga saccharolityca</em> gen. nov., sp. nov. (= DSMZ 109061<sup>T</sup> =JCM 32930<sup>T</sup> =VKM B 3262<sup>T</sup>). Furthermore, phylogenetic and phenotypic parameters of <em>N. saccharolityca</em> and <em>C. proteinivorum</em> gen. nov., sp. nov., strain Omega<sup>T</sup> (=JCM 31662<sup>T</sup>, =UNIQEM U979<sup>T</sup>), make it possible to include them into a new family with a proposed designation <em>Cyclonatronaceae</em> fam. nov..</p></div>","PeriodicalId":3,"journal":{"name":"ACS Applied Electronic Materials","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Natronogracilivirga saccharolytica gen. nov., sp. nov. and Cyclonatronum proteinivorum gen. nov., sp. nov., haloalkaliphilic organotrophic bacteroidetes from hypersaline soda lakes forming a new family Cyclonatronaceae fam. nov. in the order Balneolales\",\"authors\":\"Tatjana N. Zhilina , Dimitry Y. Sorokin , Stepan V. Toshchakov , Ilya V. Kublanov , Daria G. Zavarzina\",\"doi\":\"10.1016/j.syapm.2023.126403\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span><span>Two heterotrophic bacteroidetes strains were isolated as satellites from autotrophic enrichments inoculated with samples from hypersaline soda lakes in southwestern </span>Siberia. Strain Z-1702</span><sup>T</sup><span> is an obligate anaerobic fermentative saccharolytic bacterium from an iron-reducing enrichment culture, while </span><em>Ca</em>. Cyclonatronum proteinivorum Omega<sup>T</sup><span> is an obligate aerobic proteolytic microorganism<span> from a cyanobacterial enrichment. Cells of isolated bacteria are characterized by highly variable morphology. Both strains are chloride-independent moderate salt-tolerant obligate alkaliphiles<span> and mesophiles. Strain Z-1702</span></span></span><sup>T</sup><span><span><span> ferments glucose, maltose, fructose, </span>mannose, </span>sorbose<span>, galactose<span>, cellobiose, </span></span></span><em>N</em><span><span>-acetyl-glucosamine and alpha-glucans, including starch, glycogen, dextrin, and </span>pullulan. Strain Omega</span><sup>T</sup><span> is strictly proteolytic utilizing a range of proteins and peptones<span>. The main polar lipid fatty acid in both strains is iso-C</span></span><sub>15:0</sub>, while other major components are various C<sub>16</sub> and C<sub>17</sub><span> isomers. According to pairwise sequence alignments using BLAST </span><em>Gracilimonas</em><span> was the nearest cultured relative to both strains (<90% of 16S rRNA gene sequence identity). Phylogenetic analysis placed strain Z-1702</span><sup>T</sup> and strain Omega<sup>T</sup> as two different genera in a deep-branching clade of the new family level within the order <em>Balneolales</em> with genus. Based on physiological characteristics and phylogenetic position of strain Z-1702<sup>T</sup> it was proposed to represent a novel genus and species <em>Natronogracilivirga saccharolityca</em> gen. nov., sp. nov. (= DSMZ 109061<sup>T</sup> =JCM 32930<sup>T</sup> =VKM B 3262<sup>T</sup>). Furthermore, phylogenetic and phenotypic parameters of <em>N. saccharolityca</em> and <em>C. proteinivorum</em> gen. nov., sp. nov., strain Omega<sup>T</sup> (=JCM 31662<sup>T</sup>, =UNIQEM U979<sup>T</sup>), make it possible to include them into a new family with a proposed designation <em>Cyclonatronaceae</em> fam. nov..</p></div>\",\"PeriodicalId\":3,\"journal\":{\"name\":\"ACS Applied Electronic Materials\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2023-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Electronic Materials\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0723202023000127\",\"RegionNum\":3,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, ELECTRICAL & ELECTRONIC\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Electronic Materials","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0723202023000127","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
引用次数: 1
摘要
从西伯利亚西南部高盐苏打湖的自养富集物中分离出两株异养拟杆菌作为卫星。菌株Z-1702T是一种来自铁还原富集培养基的专性厌氧发酵解糖细菌，而Ca。嗜蛋白环钠菌OmegaT是一种来源于蓝藻富集的专性好氧蛋白水解微生物。分离的细菌的细胞具有高度可变的形态特征。这两个菌株都是不依赖氯化物的中度耐盐专性嗜碱菌和嗜中温菌。菌株Z-1702T发酵葡萄糖、麦芽糖、果糖、甘露糖、山梨糖、半乳糖、纤维二糖、N-乙酰基-葡糖胺和α-葡聚糖，包括淀粉、糖原、糊精和支链淀粉。菌株OmegaT利用一系列蛋白质和蛋白胨进行严格的蛋白水解。两个菌株中的主要极性脂质脂肪酸是异C15:0，而其他主要成分是各种C16和C17异构体。根据使用BLAST的成对序列比对，相对于两种菌株而言，薄单胞菌是最接近培养的（＜16S rRNA基因序列同一性的90%）。系统发育分析将菌株Z-1702T和菌株OmegaT作为两个不同的属，放在Balneolales目新科级的深支支支中。根据菌株Z-1702T的生理特性和系统发育位置，提出了代表一个新属和新种的新菌株（=DSMZ 109061T=JCM 32930T=VKM B 3262T）。此外，N.saccharolityca和C.proteinivorum gen.nov.，sp.nov.、菌株OmegaT（=JCM 31662T，=UNIQEM U979T）的系统发育和表型参数使它们有可能被纳入一个新的家族，命名为环钠藻科fam。十一月
Natronogracilivirga saccharolytica gen. nov., sp. nov. and Cyclonatronum proteinivorum gen. nov., sp. nov., haloalkaliphilic organotrophic bacteroidetes from hypersaline soda lakes forming a new family Cyclonatronaceae fam. nov. in the order Balneolales
Two heterotrophic bacteroidetes strains were isolated as satellites from autotrophic enrichments inoculated with samples from hypersaline soda lakes in southwestern Siberia. Strain Z-1702T is an obligate anaerobic fermentative saccharolytic bacterium from an iron-reducing enrichment culture, while Ca. Cyclonatronum proteinivorum OmegaT is an obligate aerobic proteolytic microorganism from a cyanobacterial enrichment. Cells of isolated bacteria are characterized by highly variable morphology. Both strains are chloride-independent moderate salt-tolerant obligate alkaliphiles and mesophiles. Strain Z-1702T ferments glucose, maltose, fructose, mannose, sorbose, galactose, cellobiose, N-acetyl-glucosamine and alpha-glucans, including starch, glycogen, dextrin, and pullulan. Strain OmegaT is strictly proteolytic utilizing a range of proteins and peptones. The main polar lipid fatty acid in both strains is iso-C15:0, while other major components are various C16 and C17 isomers. According to pairwise sequence alignments using BLAST Gracilimonas was the nearest cultured relative to both strains (<90% of 16S rRNA gene sequence identity). Phylogenetic analysis placed strain Z-1702T and strain OmegaT as two different genera in a deep-branching clade of the new family level within the order Balneolales with genus. Based on physiological characteristics and phylogenetic position of strain Z-1702T it was proposed to represent a novel genus and species Natronogracilivirga saccharolityca gen. nov., sp. nov. (= DSMZ 109061T =JCM 32930T =VKM B 3262T). Furthermore, phylogenetic and phenotypic parameters of N. saccharolityca and C. proteinivorum gen. nov., sp. nov., strain OmegaT (=JCM 31662T, =UNIQEM U979T), make it possible to include them into a new family with a proposed designation Cyclonatronaceae fam. nov..
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