CRISPR/Cas9基因编辑对水稻三个多胺摄取转运蛋白基因的破坏赋予其对除草剂百草枯的耐受性

IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Yu-Shu Lyu, Li-Miao Cao, Wen-Qian Huang, Jian-Xiang Liu, Hai-Ping Lu
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引用次数: 10

摘要

杂草是一种主要的生物制约因素,可导致作物产量的急剧下降。除草剂技术作为最具成本效益的杂草控制措施已被农民广泛使用,迫切需要制定新的策略来提高植物对除草剂的耐受性。基于CRISPR/Cas9的基因组编辑工具已被用于与作物改良农业技术相关的各种应用。在这里,我们在水稻中鉴定了三个与拟南芥AtRMV1同源的多胺摄取转运蛋白(PUT)基因。我们成功地证明了OsPUT1/2/3的CRISPR/Cas9靶向突变大大提高了水稻对百草枯的抗性,而没有明显的产量损失。因此,对这些基因座的操作对未来生产抗除草剂的转基因水稻具有重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Disruption of three polyamine uptake transporter genes in rice by CRISPR/Cas9 gene editing confers tolerance to herbicide paraquat

Disruption of three polyamine uptake transporter genes in rice by CRISPR/Cas9 gene editing confers tolerance to herbicide paraquat

Weeds are a major biotic constraint that can cause dramatic crop production losses. Herbicide technology has been widely used by farmers as the most cost-effective weed control measure, and development of new strategy to improve herbicide tolerance in plants is urgently needed. The CRISPR/Cas9-based genome editing tool has been used in diverse applications related to agricultural technology for crop improvement. Here we identified three polyamine uptake transporter (PUT) genes in rice that are homologous to the Arabidopsis AtRMV1. We successfully demonstrate that CRISPR/Cas9-targeted mutagenesis of OsPUT1/2/3 greatly improves paraquat resistance in rice without obvious yield penalty. Therefore, manipulation of these loci could be valuable for producing transgene-free rice with improved herbicide resistance in future.

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CiteScore
7.70
自引率
2.80%
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