O.S. Nakahara , S. Hoshino-Shimizu , Y.I. Yamamoto , M.E. Camargo
{"title":"使用过氧化物酶标记的刚地弓形虫抗原(Alk-Toxo)进行酶免疫测定(IgM cap dot EIA)诊断急性弓形虫病的评价","authors":"O.S. Nakahara , S. Hoshino-Shimizu , Y.I. Yamamoto , M.E. Camargo","doi":"10.1016/0888-0786(95)93030-F","DOIUrl":null,"url":null,"abstract":"<div><p>An IgM capture enzyme immunoassay (IgM cap dot EIA) using a peroxidase-labelled <em>Toxoplasma gondii</em> antigen was standardized and assessed for the diagnosis of acute toxoplasmosis. This assay appeared to detect mostly IgM antibodies to <em>Toxoplasma</em> antigenic bands of 30, 27, 24, 22 and 18-17 kDa. In the study of serum samples (416) from patients with acute and chronic toxoplasmosis, from patients with unrelated diseases and from clinically healthy individuals, the IgM cap dot EIA showed diagnostic features similar to those of IgM capture enzyme-linked immunosorbent assay (ELISA) and IgM immunofluorescence test. Nevertheless, only this assay gave clear-cut results.</p></div>","PeriodicalId":101161,"journal":{"name":"Serodiagnosis and Immunotherapy in Infectious Disease","volume":"7 1","pages":"Pages 40-44"},"PeriodicalIF":0.0000,"publicationDate":"1995-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0888-0786(95)93030-F","citationCount":"1","resultStr":"{\"title\":\"Evaluation of an enzyme immunoassay (IgM cap dot EIA) using a peroxidase-labelled Toxoplasma gondii antigen (Alk-Toxo) for the diagnosis of acute toxoplasmosis\",\"authors\":\"O.S. Nakahara , S. Hoshino-Shimizu , Y.I. Yamamoto , M.E. Camargo\",\"doi\":\"10.1016/0888-0786(95)93030-F\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>An IgM capture enzyme immunoassay (IgM cap dot EIA) using a peroxidase-labelled <em>Toxoplasma gondii</em> antigen was standardized and assessed for the diagnosis of acute toxoplasmosis. This assay appeared to detect mostly IgM antibodies to <em>Toxoplasma</em> antigenic bands of 30, 27, 24, 22 and 18-17 kDa. In the study of serum samples (416) from patients with acute and chronic toxoplasmosis, from patients with unrelated diseases and from clinically healthy individuals, the IgM cap dot EIA showed diagnostic features similar to those of IgM capture enzyme-linked immunosorbent assay (ELISA) and IgM immunofluorescence test. Nevertheless, only this assay gave clear-cut results.</p></div>\",\"PeriodicalId\":101161,\"journal\":{\"name\":\"Serodiagnosis and Immunotherapy in Infectious Disease\",\"volume\":\"7 1\",\"pages\":\"Pages 40-44\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1995-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0888-0786(95)93030-F\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Serodiagnosis and Immunotherapy in Infectious Disease\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/088807869593030F\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Serodiagnosis and Immunotherapy in Infectious Disease","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/088807869593030F","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Evaluation of an enzyme immunoassay (IgM cap dot EIA) using a peroxidase-labelled Toxoplasma gondii antigen (Alk-Toxo) for the diagnosis of acute toxoplasmosis
An IgM capture enzyme immunoassay (IgM cap dot EIA) using a peroxidase-labelled Toxoplasma gondii antigen was standardized and assessed for the diagnosis of acute toxoplasmosis. This assay appeared to detect mostly IgM antibodies to Toxoplasma antigenic bands of 30, 27, 24, 22 and 18-17 kDa. In the study of serum samples (416) from patients with acute and chronic toxoplasmosis, from patients with unrelated diseases and from clinically healthy individuals, the IgM cap dot EIA showed diagnostic features similar to those of IgM capture enzyme-linked immunosorbent assay (ELISA) and IgM immunofluorescence test. Nevertheless, only this assay gave clear-cut results.
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