有机凝胶介导的nisin和5-氟尿嘧啶的共同递送:一种对抗皮肤癌的协同方法。

IF 3 4区医学 Q2 CHEMISTRY, APPLIED

Journal of microencapsulation Pub Date : 2022-11-01 DOI:10.1080/02652048.2022.2149871

Jasleen Kaur, Kaisar Raza, Simran Preet

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引用次数: 0

摘要

目的:研究乳酸链球菌素和5-氟尿嘧啶单纳米联合局部治疗皮肤癌的方法。方法:将Nisin和5-氟尿嘧啶包封在有机凝胶体系(NF-OG)中，考察其形态、理化性质、细胞毒性、包封和释放性能。从肿瘤统计学、组织结构、TUNEL和M1/M2巨噬细胞等方面评价NF-OG对DMBA/TPA小鼠皮肤癌的作用。结果:优化后的NF-OG配方粒径为185.1±11.24 nm, zeta电位为-7.93±0.60 mV，具有良好的载药量和时间释放性能。NF-OG治疗改善了nisin和5-FU对B16-F10细胞的细胞毒性，与未治疗组(490.482 mm3)相比，治疗组肿瘤体积显著减少(84.983 mm3)，并伴有组织结构的恢复和巨噬细胞的复极化。结论:该研究产生了一种具有强大抗癌活性的有前途的递送系统，并为进一步在临床应用奠定了基础。

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Organogel mediated co-delivery of nisin and 5-fluorouracil: a synergistic approach against skin cancer.

Aim: The present study aimed to develop topical combinatorial therapy of nisin and 5-fluorouracil in a single nanosized formulation against skin cancer.

Methods: Nisin and 5-fluorouracil were encapsulated in an organogel system (NF-OG) and investigated for morphology, physicochemical properties, cytotoxicity, encapsulation and release. NF-OG was evaluated against DMBA/TPA murine skin cancer in terms of tumour statistics, histoarchitecture, TUNEL and M1/M2 macrophages.

Results: The optimised NF-OG formulation exhibited particle size of 185.1 ± 11.24 nm, zeta potential of -7.93 ± 0.60 mV, offered substantial drug loading and temporal release. NF-OG therapy led to improved cytotoxicity of nisin and 5-FU against B16-F10 cells, significant decrease in tumour volume (84.983 mm³) in treated group as compared to untreated group (490.482 mm³) accompanied by restoration of histoarchitecture and repolarization of macrophages.

Conclusion: The study yielded a promising delivery system exhibiting potent anticancer activity and forms the bases for further applications in clinical settings.

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来源期刊

Journal of microencapsulation 工程技术-工程：化工

CiteScore

6.30

自引率

2.60%

发文量

审稿时长

3 months

期刊介绍： The Journal of Microencapsulation is a well-established, peer-reviewed journal dedicated to the publication of original research findings related to the preparation, properties and uses of individually encapsulated novel small particles, as well as significant improvements to tried-and-tested techniques relevant to micro and nano particles and their use in a wide variety of industrial, engineering, pharmaceutical, biotechnology and research applications. Its scope extends beyond conventional microcapsules to all other small particulate systems such as self assembling structures that involve preparative manipulation. The journal covers: Chemistry of encapsulation materials Physics of release through the capsule wall and/or desorption from carrier Techniques of preparation, content and storage Many uses to which microcapsules are put.