鱼藤酮诱导的实验性帕金森大鼠的行为反应特征及脑内isatin结合蛋白谱。

Q3 Biochemistry, Genetics and Molecular Biology
I G Kapitsa, L Sh Kazieva, N E Vavilov, V G Zgoda, A T Kopylov, A E Medvedev, O A Buneeva
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引用次数: 0

摘要

神经毒素鱼tenone和1-甲基-4-苯基-1,2,3,6-四氢吡啶(МPTP)用于动物帕金森病模型(PD)。它们诱导线粒体呼吸链功能障碍,从而导致多巴胺能(DA)神经元变性。鱼藤酮模型的优势在于鱼藤酮能够引起神经退行性变,表现出与PD相似的症状和分子生物学特征。Isatin (indoldione-2,3)是一种内源性调节剂,存在于人和动物的组织和生物体液中。它表现出广泛的生物活性,由许多isatin结合蛋白介导。在这项工作中,我们研究了帕金森综合征(PS)大鼠的行为反应和脑isatin结合蛋白的特征,并与mptp诱导的小鼠帕金森病的相应参数进行了比较。全身注射鱼藤酮引起的严重PS与注射MPTP的效果相当。伴有动物体重明显减轻、死亡、运动减少、肌肉僵硬和姿势不稳定。鱼藤酮与MPTP致PS的致病基础相同,但其作用的分子机制不同。在鱼藤酮诱导的PS情况下,无论是在神经毒素注射后(27),还是在MPTP注射后一周(14),对照大鼠和PS大鼠(146)共有的isatin结合蛋白池明显超过对照大鼠和MPTP诱导的PS小鼠的共有蛋白池(14)。比较mptp诱导的PS和鱼藤酮诱导的PS动物的isatin结合蛋白特异性(与对照动物相比),发现这两种PD模型的常见蛋白完全缺失。值得注意的是,这两种神经毒素都特别影响参与信号传递和酶活性调节的蛋白质。鱼藤酮注射后isatin结合蛋白谱的变化表明,神经保护剂isatin对鱼藤酮模型PD也有积极影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Characteristics of behavioral reactions and the profile of brain isatin-binding proteins of rats with the rotenone-induced experimental parkinsonism].

The neurotoxins rotenone and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (МPTP) are used for modeling Parkinson's disease in animals (PD). They induce the mitochondrial respiratory chain dysfunction, which leads to the dopaminergic (DA) neuron degeneration. The advantage of the rotenone model consists in ability of rotenone to cause neurodegeneration showing symptoms and molecular biological characteristics similar to those of PD. Isatin (indoldione-2,3) is an endogenous regulator found in tissues and biological fluids of humans and animals. It exhibits a broad range of biological activity mediated by numerous isatin-binding proteins. In this work we have investigated behavioral reactions and profiles of brain isatin-binding proteins of rats with Parkinson's syndrome (PS) in comparison with the corresponding parameters of MPTP-induced Parkinsonism in mice. Systemic injection of rotenone caused severe PS comparable with the effect of MPTP injection. It was accompanied by significant body weight loss, death, oligokinesia, muscular rigidity, and postural instability of animals. In spite of the same pathogenic basis of PS caused by rotenone and MPTP, the molecular mechanisms of their action differ. In the case of rotenone-induced PS, the pool of isatin-binding proteins common of the control rats and the rats with PS (146) significantly exceeded the pool of the common proteins of control mice and mice with PS induced by MPTP, whether right after neurotoxin injection (27), or (all the more) in a week after the MPTP injection (14). The comparison of isatin-binding proteins specific of the animals with MPTP-induced PS and with the rotenone-induced PS (as compared with the control animals) revealed total absence of proteins common of these two models of PD. It is to be noted that both neurotoxins particularly affected the proteins participating in the signal transmission and enzyme activity regulation. The changes of the profile of isatin-binding proteins in response to the injection of rotenone suggest that the neuroprotector isatin could also influence positively in the case of the rotenone model of PD.

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来源期刊
Biomeditsinskaya khimiya
Biomeditsinskaya khimiya Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
1.30
自引率
0.00%
发文量
49
期刊介绍: The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).
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