黄曲霉人工侵染玉米籽粒贮藏过程中黄曲霉毒素产生的抑制

Sally Ibrahim Abd Elfatah, M. Abdel-Kader, N. S. El-Mougy, K. M. Soliman
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引用次数: 1

摘要

玉米是埃及的重要作物之一。黄曲霉毒素(AFs)是黄曲霉(Aspergillus flavus)最主要的致癌化合物,对人类和动物的健康造成危害。本研究旨在通过使用不同浓度的天然物质(康乃馨油、柠檬草油、蜂胶和蜂蜡)和化学物质(水杨酸和山梨酸钾)对玉米籽粒抑制黄曲霉毒素的产生。利用马铃薯葡萄糖琼脂(Potato Dextrose Agar, PDA)从当地玉米籽粒中分离到黄曲霉菌株,并检测其在椰子琼脂培养基上产生黄曲霉毒素的能力。分别用不同浓度的康乃馨油、柠檬草油、水杨酸和山梨酸钾(0.25、0.5、1.0、2.0、4.0和6.0%)和蜂胶和蜂蜡浓度分别为1%和4%的浓度分别处理100g无菌玉米,接种黄曲霉,在28±2℃条件下保存30 d。采用高效液相色谱法测定AFs的还原量。所有被试物质均对贮藏玉米中黄曲霉产生AFs有积极的抑制作用。当浓度为0.25%时,黄曲霉毒素B1和B2 (AFB1和AFB2)的产量分别降至93%和99%左右。在浓度为2% ~ 6%的条件下,柠檬草精油对afb1和AFB2的产生几乎完全抑制(分别为99.12% ~ 99.98%和99.98% ~ 99.99%)。与对照相比,山梨酸钾和水杨酸(0.25% ~ 6%)对控制玉米籽粒黄曲霉毒素的产生有显著效果。而蜂胶和蜂蜡对保护玉米籽粒免受真菌侵害的作用最为显著。天然物质康乃馨油、柠檬草油、蜂蜡和蜂胶在低浓度下对黄曲霉毒素产生的活性作用高于水杨酸,且对人体更安全。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
SUPPRESSION OF AFLATOXINS PRODUCTION IN ARTIFICIALLY INFESTED MAIZE GRAINS WITH ASPERGILLUS FLAVUS DURING STORAGE CONDITIONS
Maize is the one of important crops in Egypt. Aflatoxins (AFs) are the foremost cancer present compounds by Aspergillus flavus (A. flavus) and cause health risks to human and animals. This study aimed to suppression of aflatoxins production by A. flavus by using different concentrations of natural substances (carnation oil, lemongrass oil, propolis and beewax) and chemical substances (salicylic acid and potassium sorbate) on maize grains. The strains of A. flavus were isolated from local maize grains on Potato Dextrose Agar (PDA) and detect its ability of aflatoxins production on coconut agar media. Samples (100g) of sterilized maize grains were treated individually with different concentration of carnation oil, lemongrass oil, salicylic acid and potassium sorbate (0.25, 0.5, 1.0, 2.0, 4.0 and 6.0%) and at concentration 1% and 4% for propolis and beeswax each, then inoculated with A. flavus and stored for 30 days at 28±2˚C. AFs reduction was determined by using High Performance liquid Chromatography (HPLC). All the tested substances had active effect in inhibition of AFs production by A. flavus in stored maize grains. The production of Aflatoxin B1 and B2 (AFB1 and AFB2) was decreased to about 93% and 99% at concentration of 0.25% carnation oil. Lemongrass oil almost completely inhibited AFB1and AFB2 production (99.12% - 99.98% and 99.98% -99.99%, respectively) at concentration of 2% - 6%. Potassium sorbate and salicylic acid (0.25%- 6%) that were significantly effective controlling aflatoxins production on maize grains compared with control. While, the propolis and beewax found to be the most active to protect maize grains against fungi. Natural substances, carnation oil, lemongrass oil, beewax and propolis had higher active effect at low concentration on aflatoxin production more than salicylic acid and more safe for human used.
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