Mohammad Dehghanizadeh Baghdadabad, Nima Naderi, Vahideh Atabaki, Mohammad Ali Faramarzi, Maryam Tabarzad, Tahereh Hosseinabadi
{"title":"部分纯化的无细胞培养基多糖馏分和 PCST5 螺旋藻生物质的镇痛和抗炎作用。","authors":"Mohammad Dehghanizadeh Baghdadabad, Nima Naderi, Vahideh Atabaki, Mohammad Ali Faramarzi, Maryam Tabarzad, Tahereh Hosseinabadi","doi":"10.5812/ijpr-136661","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong><i>Spirulina</i> is a cyanobacteria species containing various bioactive compounds. <i>Spirulina</i> is a known source of nutrients in some traditional diets. Different activities have been reported for various extracts of <i>S. platensis</i>.</p><p><strong>Objectives: </strong>In this study, the polysaccharide content of culture media and biomass extract of one species of <i>Spirulina</i> was partially purified, and its analgesic and anti-inflammatory effects were evaluated.</p><p><strong>Methods: </strong><i>Spirulina platensis</i> PCST5 was cultured in a sterile Zarouk medium at 27°C and 16/8h of light/ dark exposure cycle for 25 days. Then, the polysaccharide content of biomass and cell-free culture medium samples (BPSs and CFPSs, respectively) was partially purified. The analgesic and anti-inflammatory effects were evaluated using animal models.</p><p><strong>Results: </strong><i>16S rRNA</i> gene analysis confirmed that the organism was genetically similar to <i>Spirulina platensis</i>. The CFPSs (30 and 100 mg/kg) and BPSs (30 mg/kg) significantly reduced pain-related behaviors in rats. Similarly, all samples could significantly reduce carrageenan-induced paw inflammation volume compared with the control group. Our results suggest <i>Spirulina</i>'s polysaccharide fractions (CFPSs and BPSs) had significant analgesic and anti-inflammatory effects.</p><p><strong>Conclusions: </strong>Since <i>Spirulina</i> is a readily available source of bioactive compounds, finding such potent anti-inflammatory and anti-nociceptive compounds can provide promising leads for novel drug development.</p>","PeriodicalId":12371,"journal":{"name":"Foreign Affairs","volume":"41 1","pages":"e136661"},"PeriodicalIF":6.3000,"publicationDate":"2023-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10728851/pdf/","citationCount":"0","resultStr":"{\"title\":\"The Analgesic and Anti-inflammatory Effects of Partially Purified Polysaccharide Fractions of Cell-free Medium and Biomass of <i>Spirulina platensis</i> PCST5.\",\"authors\":\"Mohammad Dehghanizadeh Baghdadabad, Nima Naderi, Vahideh Atabaki, Mohammad Ali Faramarzi, Maryam Tabarzad, Tahereh Hosseinabadi\",\"doi\":\"10.5812/ijpr-136661\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong><i>Spirulina</i> is a cyanobacteria species containing various bioactive compounds. <i>Spirulina</i> is a known source of nutrients in some traditional diets. Different activities have been reported for various extracts of <i>S. platensis</i>.</p><p><strong>Objectives: </strong>In this study, the polysaccharide content of culture media and biomass extract of one species of <i>Spirulina</i> was partially purified, and its analgesic and anti-inflammatory effects were evaluated.</p><p><strong>Methods: </strong><i>Spirulina platensis</i> PCST5 was cultured in a sterile Zarouk medium at 27°C and 16/8h of light/ dark exposure cycle for 25 days. Then, the polysaccharide content of biomass and cell-free culture medium samples (BPSs and CFPSs, respectively) was partially purified. The analgesic and anti-inflammatory effects were evaluated using animal models.</p><p><strong>Results: </strong><i>16S rRNA</i> gene analysis confirmed that the organism was genetically similar to <i>Spirulina platensis</i>. The CFPSs (30 and 100 mg/kg) and BPSs (30 mg/kg) significantly reduced pain-related behaviors in rats. Similarly, all samples could significantly reduce carrageenan-induced paw inflammation volume compared with the control group. Our results suggest <i>Spirulina</i>'s polysaccharide fractions (CFPSs and BPSs) had significant analgesic and anti-inflammatory effects.</p><p><strong>Conclusions: </strong>Since <i>Spirulina</i> is a readily available source of bioactive compounds, finding such potent anti-inflammatory and anti-nociceptive compounds can provide promising leads for novel drug development.</p>\",\"PeriodicalId\":12371,\"journal\":{\"name\":\"Foreign Affairs\",\"volume\":\"41 1\",\"pages\":\"e136661\"},\"PeriodicalIF\":6.3000,\"publicationDate\":\"2023-07-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10728851/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Foreign Affairs\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.5812/ijpr-136661\",\"RegionNum\":2,\"RegionCategory\":\"社会学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q1\",\"JCRName\":\"INTERNATIONAL RELATIONS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Foreign Affairs","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.5812/ijpr-136661","RegionNum":2,"RegionCategory":"社会学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"INTERNATIONAL RELATIONS","Score":null,"Total":0}
The Analgesic and Anti-inflammatory Effects of Partially Purified Polysaccharide Fractions of Cell-free Medium and Biomass of Spirulina platensis PCST5.
Background: Spirulina is a cyanobacteria species containing various bioactive compounds. Spirulina is a known source of nutrients in some traditional diets. Different activities have been reported for various extracts of S. platensis.
Objectives: In this study, the polysaccharide content of culture media and biomass extract of one species of Spirulina was partially purified, and its analgesic and anti-inflammatory effects were evaluated.
Methods: Spirulina platensis PCST5 was cultured in a sterile Zarouk medium at 27°C and 16/8h of light/ dark exposure cycle for 25 days. Then, the polysaccharide content of biomass and cell-free culture medium samples (BPSs and CFPSs, respectively) was partially purified. The analgesic and anti-inflammatory effects were evaluated using animal models.
Results: 16S rRNA gene analysis confirmed that the organism was genetically similar to Spirulina platensis. The CFPSs (30 and 100 mg/kg) and BPSs (30 mg/kg) significantly reduced pain-related behaviors in rats. Similarly, all samples could significantly reduce carrageenan-induced paw inflammation volume compared with the control group. Our results suggest Spirulina's polysaccharide fractions (CFPSs and BPSs) had significant analgesic and anti-inflammatory effects.
Conclusions: Since Spirulina is a readily available source of bioactive compounds, finding such potent anti-inflammatory and anti-nociceptive compounds can provide promising leads for novel drug development.
期刊介绍：
Founded in 1922, Foreign Affairs is a prominent American magazine that focuses on international relations and U.S. foreign policy. It is published by the Council on Foreign Relations, an esteemed nonpartisan think tank and membership organization dedicated to analyzing U.S. foreign policy and global affairs. While the print magazine is released every two months, the website offers daily articles and publishes anthologies every other month.
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