无花果树的抗氧化和细胞毒活性研究轧机。水果中提取

M. E. Okur, N. Karakaş, A. Karadaǧ, Damla Uludağ, D. Polat
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引用次数: 4

摘要

据称,无花果树果实具有几种有益健康的特性。本研究旨在测定ofi水果甲醇提取物的植物化学成分,并比较其抗氧化活性和细胞活力。通过DPPH、ABTS等体外实验评价其抗氧化活性。并进行了LC-MS和HPLC分析。通过荧光素酶系统(Cell TiterGlo)测定活细胞,研究了ofi果提取物对U87-MG(多形性胶质母细胞瘤)、HT-29(结肠癌)和人原代成纤维细胞的活性。Promega;美国)。结果表明,ofi水果是抗氧化物质的来源。此外,该提取物还具有丰富的酚类黄酮含量。液相色谱-质谱和高效液相色谱分析发现槲皮素。HT-29和U87细胞系的细胞死亡呈浓度依赖性(1-10mg/ml)显著增加。在这些细胞系中,即使是最低剂量(1mg/ml), HT-29对ofi果处理的反应也更为敏感。结果表明,枸杞果具有显著的抗氧化能力,对多种癌细胞具有较强的细胞毒活性。因此,ofi水果可能具有化学预防潜力,可以进一步研究以阐明其细胞毒性。您可以将本文引用为:Okur ME, Karakas N, Karadag AE, Uludag D, Polat DC(2019)。无花果树的抗氧化和细胞毒活性研究轧机。水果中提取。IstanbulJPharm 10.26650/ istanbuljpharma .2019.19035。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Investigation of Antioxidant and Cytotoxic Activities of Opuntia ficus-indica (L.) Mill. Fruit extract
DOI : 10.26650/IstanbulJPharm.2019.19035 Opuntia ficus-indica fruits are claimed to possess several health-beneficial properties. This study was carried out to determine the phytochemicals as well as to compare antioxidant activity and cell viability of the methanol extract of OFI-fruits. Antioxidant activity was evaluated by various in vitro tests such as DPPH, ABTS. Furthermore LC-MS and HPLC analysis were performed. The activity of the OFI-fruit extract on viability of U87-MG (glioblastoma multiform), HT-29 (colon cancer) and human primary fibroblast cells has been investigated by determining viable cells on a luciferase based system (Cell TiterGlo; Promega;USA). The results showed that OFI-fruits were source of antioxidant compounds. Moreover, the extract was found to have rich phenolic-flavonoid contents. Quercetin was found in the extract according to LC-MS and HPLC studies. The remarkable increased cell death in the HT-29 and U87 cell lines was detected in a concentration-dependent manner (1-10mg/ml). Among these cell lines, HT-29 responded to OFI-fruit treatment more sensitively to even the lowest dose (1mg/ml). The results of this study showed the OFI-fruit has significant antioxidant capacity and leads to strong cytotoxic activity on various cancer cells. Therefore, it can be suggested that OFI-fruits may have chemo-preventive potential and can be studied further to clarify cytotoxic abilities. You may cite this article as : Okur ME, Karakas N, Karadag AE, Uludag D, Polat DC (2019). Investigation of Antioxidant and Cytotoxic Activities of Opuntia ficus-indica (L.) Mill. Fruit extract. Istanbul J Pharm 10.26650/IstanbulJPharm.2019.19035.
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