大颗粒胶体银在灵长类脊椎动物中的多器官致畸后遗症

P. J. Prakash, Singh Royana, Pani Sankarsan
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引用次数: 1

摘要

背景:在这个最新的,更新的全球消费者产品舞台上,大多数较大的银纳米颗粒(20至100纳米范围)的日常应用在临床医学和诊断中起到抗病毒和抗寄生虫剂的作用,这是一个积极的反馈。然而,大尺寸纳米银粒子对人体、动物和灵长类脊椎动物的主要负反馈是多系统致畸性。材料与方法:研究大尺寸聚乙烯软锰酸酯包被、硼氢化钠稳定纳米银的致畸作用。为此,我们将其胶体溶液形式以纳米银-71-1900.8纳米范围,以14、19 mg/kg/b.w的剂量反复灌胃的方式注射给不同繁殖群体的瑞士白化雌性妊娠小鼠(每组10只)。/d,假对照用阴离子双蒸馏水处理,妊娠期7-18天(12天)。死亡动物麻醉,第19天腹开腹手术。收集胎儿进行睁眼和手晶状体形态学验证,定位各种致畸异常。结果:治疗组胎儿出现多系统致畸,如并指畸形、少指畸形、多指畸形、脑膜脊髓膨出、闭合型神经管畸形、扁平肋骨等骨骼畸形、缺骨节段或骨裂、脊柱侧凸等椎体畸形、缺长骨或其部分或发育不全甚至融合不全。我们还发现了中枢神经系统的畸形,如大脑、海马和小脑的畸形。在组织学上观察到蜂窝状畸形和中枢神经系统细胞空泡化与氧化应激显著相关。结论:大尺寸纳米银颗粒通过增加机体氧化应激和抑制机体营养,引起多系统和中枢神经系统功能损伤及致畸后遗症。今后,为了医疗和诊断目的而使用更大的银纳米颗粒需要仔细考虑,特别是如果它涉及人体多系统和中枢神经系统的暴露,因为它可能在剂量与强度成正比的情况下引起后代的多系统致畸性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Multi-Organ Teratogenesis Sequels of Bigger Size Particles Colloidal Silver in Primate Vertebrates
Back ground: In this most recent, update global arena for consumers products most of the daily applications of bigger silver nano particles (20 to 100 nano meter range) are effected as anti-viral and anti-parasitic agents in clinical medicine and diagnosis which is a positive feedback. However, the major negative feedback of bigger size silver nano particles on human, animal and primate vertebrate body is multisystem teratogenicity focuses. Material and methods: This study was designed to investigate teratogenic effects of bigger size nano silver which is poly vinyl pyrollidone coated and sodium borohydride stabilized. For this we injected its colloidal solution form in to pregnant female Swiss albino mice of different breeding colonies (10 from each group) in repeated oral gavages manner with nanosilver-71-1900.8 nano meter range at a dose of 14, 19 mg/kg/b.w./day with sham control treated by anionic double distilled water for 7-18 gestational days (12 days). Then dead animals are then anaesthetized and operated by ventral laparotomy on day 19. Fetuses were collected for open eye and hand lens morphological verification to locate various teratogenic anomalies. Result: We found multi system teratogenicity in treated group fetuses like Syndactyly, Oligodactyly, Polydactyl, meningo myelocele, closed type neural tube deformity, skeletal deformity like flat ribs, absence of bony segments or cleft, vertebral deformity like scoliosis, absence of long bones or its parts or mal developments or even mal fusion. We also found Central nervous system teratogenic effects like malformation of cerebrum, hippocampus and cerebellum. Histological teratogenic effects are observed in form of honey comb deformity and cell vacuolization in Central nervous system were significantly correlated with oxidative stress. Conclusion: Our findings suggest that bigger size silver nano particles induce an impairment of multi system and Central nervous system functions and teratogenic sequels by increasing oxidative stress and hampering nutrition in those body parts. The use of bigger silver nano particles for medical and diagnostic purposes henceforth requires careful consideration, particularly if it involves exposure of the human multi system and Central nervous system as it may evokes multi system teratogenicity in offspring’s where dose directly proportional to intensity.
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