香蕉皮提取物的抗菌活性(摩西的例子)针对菌根葡萄球菌和大肠杆菌以及总测定类黄酮和苯酚的活性成分

N. D. Wahyuni, W. S. Rita, I. Asih
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引用次数: 3

摘要

摘要本研究旨在揭示竹黄香蕉皮提取物对金黄色葡萄球菌和大肠杆菌的抗氧化活性,并测定活性提取物中总黄酮和酚类物质的含量。采用浸渍分割法提取黄竹香蕉果皮,孔扩散法测定其抑菌活性,紫外-可见分光光度法测定黄竹香蕉总黄酮和酚类物质含量。黄香蕉皮浸渍1公斤可得粗乙醇提取物80.9173 g。粗乙醇提取液20 g,分馏得到正己烷提取液1 3758 g,乙酸乙酯提取液3 5818 g,正丁醇提取液1 0762 g。抑菌试验结果表明,10%正丁醇提取物对金黄色葡萄球菌和大肠杆菌具有较强的抑菌活性,与乙醇、乙酸乙酯和正己烷提取物相比具有较强的抑菌活性。金黄色葡萄球菌MIC值为0.5%,大肠杆菌MIC值为0.2%。正丁醇提取物中总黄酮和酚的含量分别为0.06%和0.15%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
AKTIVITAS ANTIBAKTERI EKSTRAK KULIT PISANG KEPOK KUNING (Musa paradisiaca L.) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli SERTA PENENTUAN TOTAL FLAVONOID DAN FENOL DALAM FRAKSI AKTIF
Peel of yellow kepok banana (Musa paradisiaca L). has not been used optimally, while the peel can be used as an infection medicine The aim of this study was to reveal the activity of kepok yellow banana peel extract against Staphylococcus aureus and Escherichia coli and to determine the total content of flavonoids and phenols in active extract.. Extraction peel of yellow kepok banana was done by maceration and partition method, anti bacterial activity was assayed by wells diffusion method, determination total flavonoid and phenolic contents was done by UV-Vis Spectrophotometer. Maceration of 1 kg peel of yellow banana produced 80.9173 g of crude ethanol extract. The partition of 20 g crude ethanol extract produced 1,3758 g of n-hexane extract, 3,5818 g of ethyl acetate extract, and 1,0762 g of n-butanol extract. Anti bacterial test result showed that the 10% n-butanol extract was active towards S.aureus and E.coli with strong activity compared with ethanol, ethyl acetate, and n-hexane extract. MIC value was 0.5% for S.aureus and 0,2% for E.coli bacteria. The contain total flavonoid and phenol in n-butanol extract respectively were 0.06% and 0.15%.
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