海洋沉积物中环境DNA对鱼类组成的初步研究

Tomohiro Shiragaki, Taku Inoue, Hideki Fukuda, Masayuki Ushio, Miki Kusaka, T. Okano, H. Takasu
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引用次数: 2

摘要

海洋沉积物中鱼类的环境DNA (eDNA)保存了很长时间,可以作为鱼类组合的连续代理数据。然而,鱼类eDNA的元条形码分析尚未应用于海洋沉积物。本研究将鱼类eDNA的元条形码分析应用于2011年东北太平洋沿岸地震引发海啸后采集的大池湾地震沉积物样本,以追踪过去的鱼类物种组成。在整个研究期间,我们成功地从沉积物样本中检测到17种鱼类的eDNA。前两年样本中洄游鱼类的序列读数较高,后两年样本中底栖鱼类的序列读数较高。eDNA元条形码鉴定的鱼类物种的时间变化与日本东北部太平洋沿岸目测普查鉴定的鱼类物种一致。因此,海洋沉积物中的eDNA可以用来追踪过去的鱼类物种组成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Pilot Study on Fish Species Composition Using Environmental DNA in Marine Sediment
The environmental DNA ( eDNA ) derived from fish in marine sediments is preserved for long periods and could be used as successive proxy data for fish assemblages. However, metabarcoding analysis for fish eDNA has never been applied to marine sediments. In this study, metabarcoding analysis for fish eDNA was applied to the Tohoku Earthquake sediment samples collected from Otsuchi Bay after the tsunami induced by the 2011 Earthquake off the Pacific Coast of Tohoku to track the past fish species composition. We successfully detected 17 fish eDNA from sediment samples throughout the study period. The number of sequence reads of migratory fish species was relatively high in the samples of the first two years, whereas that of demersal fish species increased in the samples of the latter two years. Temporal changes in fish species identified by eDNA metabarcoding are consistent with those in fish species identified by visual censuses in the Pacific coast of northeastern Japan. Thus, eDNA in marine sediments could be used to track the past fish species composition.
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