番木瓜叶甲醇提取物对糖尿病雄性大鼠的生育作用及降血脂活性评价

Opeyemi O. Ayodele, Ifeoluwa M. Dada, Rotimi K. Adekunle
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摘要

目的:糖尿病(DM)是一种常见的代谢紊乱,可导致其他微血管和大血管并发症。糖尿病会影响生育能力和血液凝固,还会导致血脂受损,从而增加动脉粥样硬化风险和冠状动脉疾病。本研究探讨番木瓜叶甲醇提取物对糖尿病雄性大鼠生育指标和血脂的影响。方法:雄性Wistar白化大鼠随机分为5组,每组6只。单次腹腔注射链脲佐菌素(55 mg/kg)诱导大鼠糖尿病。分别给予100、200 mg/kg木瓜甲醇提取物口服治疗糖尿病大鼠14 d。给药结束时,用分光光度法测定血浆葡萄糖浓度和血脂;在显微镜下进行精液分析,评估精子形态、活力和数量。测定大鼠的出血和凝血时间。结果:木瓜叶甲醇提取物显著(p = 0.05)降低糖尿病治疗大鼠血浆葡萄糖、总胆固醇、甘油三酯、VLDL-C、LDL-C、出血和凝血次数,治疗组HDL-C显著(p = 0.05)高于糖尿病对照组。糖尿病对照大鼠正常细胞比例(41.4±4.4%)和100 mg/kg番木瓜处理组(50.0±8.5%)较低;200 mg/kg组为47.5±9.1%,正常对照组为69.5±5.6%。精子数量也记录了类似的结果。定性植物化学筛选显示存在类固醇、蒽醌、单宁和其他生物活性化合物。结论:木瓜叶甲醇提取物具有降血糖、降血脂的作用。因此,可被认为是管理和控制糖尿病及其并发症的生物药理学药物的潜在来源。长期服用番木瓜叶可能会对雄性生育力产生负面影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Assessment of Fertility Effect and Hypolipidemic Activity of Carica papaya (Linn) Leaf Methanol Extract in Male Diabetic Rats
Aim: Diabetes mellitus (DM) is a prevalent metabolic disorder that leads to other microvascular and macrovascular complications. Diabetes affects fertility and blood clotting, and also cause impaired lipid profile thus leading to increased atherogenic risks and coronary diseases. This research investigates the effects of Carica papaya leaf methanol extract on fertility indices and lipid profile of male diabetic rats. Methodology: Male Wistar albino rats were randomly divided into five groups of six rats each. Diabetes was induced in the rats by a single intraperitoneal injection of streptozotocin (55 mg/kg). Diabetic rats were treated orally with 100 and 200 mg/kg C. papaya methanol extract for 14 days. At the end of administration, the plasma glucose concentration and lipid profile were assayed by spectrophotometric methods; seminal analysis was carried out for evaluation of morphology, motility and sperm count under the microscope. The bleeding and clotting times of the rats were also determined. Results: C. papaya leaf methanol extract caused significant (p = 0.05) reduction in plasma glucose, total cholesterol, triglycerides, VLDL-C, LDL-C, bleeding and clotting times of diabetic treated rats, while the HDL-C of treated groups were significantly (p = 0.05) elevated compared to the diabetic control. Percentage normal cells were lower in diabetic control rats (41.4±4.4%) and C. papaya treated groups (50.0±8.5% for 100 mg/kg; 47.5±9.1% for 200 mg/kg) compared with the normal control group (69.5±5.6%). Similar results were recorded for sperm count. The qualitative phytochemical screening showed the presence of steroids, anthraquinone, tannin, and other bioactive compounds. Conclusion: findings from this study indicated that C. papaya leaf methanol extract could possess hypoglycemic and hypolipidemic activities. Thus, could be considered as a potential source of bio pharmacological agent for management and control of DM and its complications. Prolonged administration of C. papaya leaves may negatively affect male fertility.
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