{"title":"复制爬虫细胞","authors":"W. Ahne","doi":"10.1111/J.1439-0450.1984.TB01344.X","DOIUrl":null,"url":null,"abstract":"Zusammenfassung \n \nDie mit einer Saline-Trypsin-Versenlosung dispergierten, von 3 Reptilienarten (Agamo stellio, Boa constrictor, Natrix natrix helvetica) abstammenden Herzzellen, liesen sich mit gutem Anzuchtserfolg mit MEM in vitro bei 25–30°C kultivieren. Nur die Gonaden der Boa constrictor erbrachten ahnliche Ergebnisse. Es konnten maximal 11 Subkulturen angelegt werden. \n \n \n \nSummary \n \nPreparation of tissue cultures from reptiles \n \n \n \nHeart cells of 3 reptiles species (Agamo stellio, Boa constrictor, Natrix natrix helvetica) obtained by a treatment with a saline trypsin versene solution grew well in MEM at 25–30°C. Gonads of the Boa constrictor could be cultivated with similar results only. The maximal subculture rate was 11. \n \n \n \nResumen \n \nLa elaboracion de cultivos celulares reptilianos \n \n \n \nCelulas cardiacas procedentes de 3 especies de reptiles (Agamo stellis, Boa constrictor, Natrix natrix helvetica), dispersadas con una solucion salina de tripsina y versenico, se pudieron cultivar in vitro con MEM a 25–30°C. Solo las gonadas de la Boa constrictor produjeron resultados similares. La tasa maxima de subcultivos era de 11.","PeriodicalId":17577,"journal":{"name":"Journal of Veterinary Medicine Series B-infectious Diseases and Veterinary Public Health","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2010-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Die Herstellung von Reptilien-Zellkulturen\",\"authors\":\"W. Ahne\",\"doi\":\"10.1111/J.1439-0450.1984.TB01344.X\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Zusammenfassung \\n \\nDie mit einer Saline-Trypsin-Versenlosung dispergierten, von 3 Reptilienarten (Agamo stellio, Boa constrictor, Natrix natrix helvetica) abstammenden Herzzellen, liesen sich mit gutem Anzuchtserfolg mit MEM in vitro bei 25–30°C kultivieren. Nur die Gonaden der Boa constrictor erbrachten ahnliche Ergebnisse. Es konnten maximal 11 Subkulturen angelegt werden. \\n \\n \\n \\nSummary \\n \\nPreparation of tissue cultures from reptiles \\n \\n \\n \\nHeart cells of 3 reptiles species (Agamo stellio, Boa constrictor, Natrix natrix helvetica) obtained by a treatment with a saline trypsin versene solution grew well in MEM at 25–30°C. Gonads of the Boa constrictor could be cultivated with similar results only. The maximal subculture rate was 11. \\n \\n \\n \\nResumen \\n \\nLa elaboracion de cultivos celulares reptilianos \\n \\n \\n \\nCelulas cardiacas procedentes de 3 especies de reptiles (Agamo stellis, Boa constrictor, Natrix natrix helvetica), dispersadas con una solucion salina de tripsina y versenico, se pudieron cultivar in vitro con MEM a 25–30°C. Solo las gonadas de la Boa constrictor produjeron resultados similares. La tasa maxima de subcultivos era de 11.\",\"PeriodicalId\":17577,\"journal\":{\"name\":\"Journal of Veterinary Medicine Series B-infectious Diseases and Veterinary Public Health\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2010-05-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Veterinary Medicine Series B-infectious Diseases and Veterinary Public Health\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1111/J.1439-0450.1984.TB01344.X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Veterinary Medicine Series B-infectious Diseases and Veterinary Public Health","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1111/J.1439-0450.1984.TB01344.X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
摘要伴随有Saline-Trypsin-Versenlosung dispergierten从3爬行动物(Agamo stellio姐们害,Natrix号Natrix脑细胞)人心脏细胞,liesen Anzuchtserfolg地处理纵使模在试管25-30培养°C .刚开始的章鱼交配带来了惊人的结果最多放了11个亚文化组织工程Summary Preparation of cultures从reptiles心cells of 3 reptiles物种(Agamo stellio姐们害,Natrix Natrix obtained海滩甲5号)有一个见称trypsin versene答案格鲁因为在纵使模在25-30°C .肚子开始模糊了目前德国文化有为Resumen La elaboracion de cultivos celulares reptilianos Celulas cardiacas procedentes de 3 especies reptiles (de Agamo stellis姐们害,Natrix Natrix号),dispersadas式过滤solucion萨利纳德tripsina y versenico就是pudieron cultivar在试管内康纵使模a 25-30°C .祝我们好运壮阔天空下的人类
Zusammenfassung
Die mit einer Saline-Trypsin-Versenlosung dispergierten, von 3 Reptilienarten (Agamo stellio, Boa constrictor, Natrix natrix helvetica) abstammenden Herzzellen, liesen sich mit gutem Anzuchtserfolg mit MEM in vitro bei 25–30°C kultivieren. Nur die Gonaden der Boa constrictor erbrachten ahnliche Ergebnisse. Es konnten maximal 11 Subkulturen angelegt werden.
Summary
Preparation of tissue cultures from reptiles
Heart cells of 3 reptiles species (Agamo stellio, Boa constrictor, Natrix natrix helvetica) obtained by a treatment with a saline trypsin versene solution grew well in MEM at 25–30°C. Gonads of the Boa constrictor could be cultivated with similar results only. The maximal subculture rate was 11.
Resumen
La elaboracion de cultivos celulares reptilianos
Celulas cardiacas procedentes de 3 especies de reptiles (Agamo stellis, Boa constrictor, Natrix natrix helvetica), dispersadas con una solucion salina de tripsina y versenico, se pudieron cultivar in vitro con MEM a 25–30°C. Solo las gonadas de la Boa constrictor produjeron resultados similares. La tasa maxima de subcultivos era de 11.
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