Sensitivity and specificity of 99mTc-UBI29-41 and 67Ga-Citrate scintigraphy imaging to discriminate infection lesion induced by Staphylococcus aureus and sterile inflammation lesion induced by Carrageenan in foot’s rat

This study was launched to evaluate the efficacy and efficiency of 99mTc-UBI29-41scintigraphy imaging to visualize the infection foci induced by staphylococcus aureus and inflammation lesions induced by carrageenan in the foot’s rat in comparison with Ga-Citrate radioisotope scintigraphy imaging. The labeling and quality control of 99mTc-UBI29-41have been performed according to the manufacturer’s instructions. A total number thirty six adult, male NMRI rats were chosen. The animals were randomly divided into two equal group’s .One group for 99mTc-UBI29-41 scintigraphy imaging and the other group for Ga-Citrate scintigraphy imaging respectively. Every group subdivided into two groups equally. Septic lesion was induced by Staphylococcus aureus due to inoculation of bacteria suspension in the foot’s rat in one group. The aseptic inflammation lesion was induced by Carrageenan in the foot’s rat in the other group. The 99mTc-UBI29-41 and Ga-Citrate radiotracer scintigraphy imaging studies have been performed to evaluate the sensitivity and specificity 99mTc-UBI29-41radiopharmaceutical for preferentially diagnosis between infection and sterile inflammation lesions. The images have been shown the uptake Ga at the infection and inflammation sites. The labeling of UBI by technetium can provide images with good quality and a shorter investigation time in comparison to Ga radioisotope imaging. The infection foci could be visualized by 99mTc-UBI2941scintigraphy imaging due to selective bonding UBI 29-41 to the negatively charged groups present on the microbial membrane due to electrostatic interaction. The inflammation sites have been observed by non-specific uptake of 99mTc-UBI29-41. Both scintigraphy imaging studies have not demonstrated preferentially diagnosis septic and aseptic inflammation lesions. The sensitivity, specificity and positive predictive value of both scintigraphy imaging techniques were 100%, 50% and 50% respectively. In spite of high sensitive of the TcUBI 29-41 scintigraphy imaging to localize the lesions, but it could not demonstrate to discriminate between septic and aseptic inflammation lesions. The other modalities must be considered for interpretation of images has obtained by Tc-UBI 29-41 scintigraphy imaging study.