小茴香黄酮对紫外线b诱导的人真皮成纤维细胞氧化应激的保护作用

J. Patwardhan, P. Bhatt
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引用次数: 0

摘要

背景:传统上,茴香籽因其抗菌、镇痛、解热、抗胀气、抗痉挛和抗雄激素活性而被使用。材料与方法:研究茴香种子黄酮对紫外线(UV)-B辐射诱导的人真皮成纤维细胞(HDF)细胞损伤和氧化应激的保护作用。结果:茴香种子富黄酮组分(FEF)具有较高的类黄酮含量和抗氧化活性,并含有标记化合物芦丁。用FEF (15-45 μg/ml)预处理HDF细胞,对uv - b诱导的细胞毒性、内源性酶抗氧化缺失、DNA氧化损伤、细胞内活性氧生成和凋亡形态学改变具有显著的保护作用。结论:本研究首次证实茴香籽FEF通过核因子e2相关因子2-抗氧化反应元件途径降低氧化应激。从茴香种子中提取的黄酮类化合物具有潜在的UV-B保护剂,可以用于对抗与氧化应激密切相关的疾病。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Protective effect of flavonoids from Foeniculum vulgare against ultraviolet-B-induced oxidative stress in human dermal fibroblasts
Background: Traditionally, Foeniculum vulgare (fennel seeds) has been used for its antimicrobial, analgesic, antipyretic, antiflatulence, antispasmodic, and antiandrogenic activities. Materials and Methods: In the present study, the protective effect of flavonoids from fennel seeds was investigated against ultraviolet (UV)-B radiation-induced cell damage and oxidative stress in human dermal fibroblast (HDF) cells. Results: Flavonoid-enriched fraction (FEF) of fennel seeds showed high flavonoid content and antioxidant potential as well as the presence of a marker compound rutin. Pretreatment of HDF cells with the FEF (15–45 μg/ml) significantly protected against UV-B-induced cytotoxicity, endogenous enzymatic antioxidant depletion, oxidative DNA damage, intracellular reactive oxygen species generation, and apoptotic morphological changes. Conclusion: The current study proved for the first time that the FEF of fennel seeds reduced oxidative stress through the nuclear factor E2-related factor 2-antioxidant response elements pathway. Flavonoids from fennel seeds have a potential as UV-B protectants and can be explored against diseases, in which oxidative stress is closely implicated.
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