Marina Indriasari, Hardinsyah, L. Kustiyah, B. Priosoeryanto, F. H. Idris
{"title":"红素B作为实验性大鼠痉挛制剂的剂量测定","authors":"Marina Indriasari, Hardinsyah, L. Kustiyah, B. Priosoeryanto, F. H. Idris","doi":"10.22606/MCMR.2019.32001","DOIUrl":null,"url":null,"abstract":"This study aimed to make the experimental rats spastic using Erythrosine B (ErB) in the correct dose. Five male Sprague-Dawley rats (T) aged 10-12 weeks with a weight of 200-250 mg were injected with ErB and doses as follows: 20 mg/kg bw for T1, 80 mg/kg bw for T2, 160 mg/kg bw for T3, 320 mg/kg bw for T4, and 400 mg/kg bw for T5. The results of 4-wk observation indicated that T1 had a rapid decrease in spasticity. T2 still experienced spasticity on day 3 with 100% muscle resistance. Its spasticity reduced by 25% within 14 days, and it still experienced spasticity with 50% muscle resistance within 28 days. T3 experienced spasticity with 100% muscle resistance until day 7. T4 and T5 died after induction. It could be concluded that the ErB dose of 80 mg/kg bw was used as a preparation for the induction of experimental rats.","PeriodicalId":91930,"journal":{"name":"Modern clinical medicine research","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2019-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Determination of the Dose of Erythrosine B as a Preparation for Inducing the Experimental Rats to Be Spastic\",\"authors\":\"Marina Indriasari, Hardinsyah, L. Kustiyah, B. Priosoeryanto, F. H. Idris\",\"doi\":\"10.22606/MCMR.2019.32001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This study aimed to make the experimental rats spastic using Erythrosine B (ErB) in the correct dose. Five male Sprague-Dawley rats (T) aged 10-12 weeks with a weight of 200-250 mg were injected with ErB and doses as follows: 20 mg/kg bw for T1, 80 mg/kg bw for T2, 160 mg/kg bw for T3, 320 mg/kg bw for T4, and 400 mg/kg bw for T5. The results of 4-wk observation indicated that T1 had a rapid decrease in spasticity. T2 still experienced spasticity on day 3 with 100% muscle resistance. Its spasticity reduced by 25% within 14 days, and it still experienced spasticity with 50% muscle resistance within 28 days. T3 experienced spasticity with 100% muscle resistance until day 7. T4 and T5 died after induction. It could be concluded that the ErB dose of 80 mg/kg bw was used as a preparation for the induction of experimental rats.\",\"PeriodicalId\":91930,\"journal\":{\"name\":\"Modern clinical medicine research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-07-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Modern clinical medicine research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.22606/MCMR.2019.32001\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Modern clinical medicine research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22606/MCMR.2019.32001","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Determination of the Dose of Erythrosine B as a Preparation for Inducing the Experimental Rats to Be Spastic
This study aimed to make the experimental rats spastic using Erythrosine B (ErB) in the correct dose. Five male Sprague-Dawley rats (T) aged 10-12 weeks with a weight of 200-250 mg were injected with ErB and doses as follows: 20 mg/kg bw for T1, 80 mg/kg bw for T2, 160 mg/kg bw for T3, 320 mg/kg bw for T4, and 400 mg/kg bw for T5. The results of 4-wk observation indicated that T1 had a rapid decrease in spasticity. T2 still experienced spasticity on day 3 with 100% muscle resistance. Its spasticity reduced by 25% within 14 days, and it still experienced spasticity with 50% muscle resistance within 28 days. T3 experienced spasticity with 100% muscle resistance until day 7. T4 and T5 died after induction. It could be concluded that the ErB dose of 80 mg/kg bw was used as a preparation for the induction of experimental rats.