The Effect of Plasma Activated Verbascoside on HIF-1A Expression as Valuable Metastasis and Angiogenesis factor in HT-29 Colon Cancer Cells

Colon cancer is one of the most common and most prevalent Neoplasma in men; adenocarcinomas make up 95 percent of all colon cancer cases. Due to the side effects of conventional treatment methods such as chemotherapy, today, attentions have been attracted to natural compounds that have less side effects and anticancer properties.

In attempting to investigate factors which may account for cancer treatment, this study raises two interrelated questions. Firstly, what is the effect of Verbascoside as a natural compound in inhibiting HIF-1α signaling pathway which is an effective route in the metastasis, angiogenesis and cell migration in the human cell line of HT-29 colon cancer; second, is it possible to enhance and raise the effect of this herbal drug by cold atmospheric pressure plasma jet?

The focus of this study will be investigating the effect of Verbascoside as a natural compound in inhibiting HIF-1α signaling pathway and also examining the effect of atmospheric pressure plasma on the function of Verbascoside.

A the first step, 29-HT cells were treated at different concentrations of verbascoside 10, 20, 30, 40, 50, 70, 100 μg / ml for 24 hours, then MTT test was used to calculate the inhibitory concentration. The colon cancer cell migration was evaluated by scratch test and HIF-1α gene expression was evaluated by Real Time PCR. The results of the MTT assay showed that verboscoside dose-dependent inhibition of cancer cells. The HT-29 colon is at a concentration of 50 μg/ml. Scratch repair tests showed that verboscoside reduced the migration of cells in a dose-dependent manner, and the results of Real Time PCR showed a decrease in the expression of HIF-1α gene expression. Cancer tumors undergo some changes in oxygen deficiency conditions, including increasing the expression of the HIF-1α gene, which in turn play an important role in activating and enhancing the expression of genes involved in metastasis, angiogenesis and migration.

At the second step of this study, verboscoside was primarily treated by cold atmospheric plasma jet then the same procedure has been taken for plasma activated verboscoside. In this study helium jet has been used.