玻璃化加热卵巢中的氧化还原反应。

F&S science Pub Date : 2024-02-01 DOI:10.1016/j.xfss.2023.11.002

Atefe Rahimi D.V.M , Ali Shahriari Ph.D. , Farid Barati Ph.D.

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引用次数: 0

摘要

背景:卵巢玻璃化是保存化疗妇女生育能力和保护珍贵或濒危物种的一种方法。然而，由不同细胞和材料组成的复杂组织的低温保存遇到了各种挑战，包括氧化应激损伤。目的:评价玻璃化牛卵巢的氧化应激指标。方法:用终浓度的乙二醇(25%)、甘油(25%)和0.5 M蔗糖玻璃化牛卵巢皮质片(1×1×1 mm3)， 48 h后，用浓度下降的蔗糖(0.5、0.25和0.125 M)加热。对卵巢进行处理，测定氧化应激的生化指标。结果:玻璃化后总抗氧化能力降低45%(结论:玻璃化过程中氧化/抗氧化平衡发生了广泛的改变。

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Redox reactions in vitrified-warmed ovary

Backgrounds

Ovary vitrification is a way for the preservation of fertility in women undergoing chemotherapy and for protecting the valuable or the endangered species. However, cryopreservation of complex tissues, which are composed of different cells and materials, encountered various challenges including oxidative stress damage.

Objectives

This study aimed to evaluate some oxidative stress indices in the vitrified bovine ovaries.

Methods

The pieces of the bovine ovarian cortex (1 × 1 × 1 mm³) were vitrified with final concentrations of ethylene glycol (25%) and glycerol (25%) and 0.5 M sucrose and then, after 48 h, were warmed with descending concentrations (0.5, 0.25, and 0.125 M) of sucrose. The ovaries were processed and some biochemical indicators of oxidative stresses were assayed.

Results

Total antioxidant capacity had a 45% decrease after vitrification (P<.0001). This reduction was associated with a 4 times increase in malondialdehyde (P=.0002) and a 53% decrease in superoxide dismutase (P=.0081). The levels of protein carbonyl in vitrified-warmed ovaries were less than in fresh ovaries (P=.0325). Regression analysis showed that the components of oxidative stress indices in vitrified tissues are different from those of fresh tissues.