剪切流作用下成肌细胞融合层的行为

S. Hashimoto, Haruki Kinoshiro, Yuta Nagasawa
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引用次数: 0

摘要

为了研究剪切应力方向对合流层中细胞活动方向的影响,在体外光学跟踪了平行或垂直于剪切流场方向的细胞的迁移和变形。下部固定培养皿与上部旋转培养皿之间形成平行壁面间的库特型剪切流。剪切应力(<2 Pa)通过调节上盘转速来设定。将成肌细胞(C2C12:小鼠成肌细胞系)在配备倒置相差显微镜的培养箱中连续剪切流培养7天至细胞融合。在延时图像中跟踪每个细胞的变形和迁移。对这些图像的分析表明,即使在合流状态下(无论细胞的长轴与剪切应力场平行还是垂直),细胞也会沿着它们的长轴变形和迁移。因此,细胞的长轴方向保持平行或垂直于剪切应力场。这一观察结果可用于改善工程肌肉组织的发育。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Behavior of a Confluent Layer of Myoblasts Under Shear Flow
To investigate the effect of shear stress direction on the direction of cell activity in a confluent layer, the migration and deformation of cells oriented parallel or perpendicular to the direction of the shear flow field were optically tracked in vitro. A Couette-type shear flow between parallel walls was formed between the lower stationary culture dish and the upper rotating disk. Shear stress (<2 Pa) was set by adjusting the rotational speed of the upper disk. Myoblasts (C2C12: mouse myoblast cell line) were cultured in an incubator equipped with an inverted phase-contrast microscope under continuous shear flow for 7 days until confluency. Deformation and migration of each cell were tracked in time-lapse images. Analysis of these images showed that cells deform and migrate along their major axis even at confluency (whether the major axis of the cell is parallel or perpendicular to the shear stress field). As a result, the orientation of the major axis of the cell remains parallel or perpendicular to the shear stress field. This observation may be used to improve the development of engineered muscle tissue.
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