骆驼发酵乳中乳酸菌的分离及对常见致病菌的抑菌活性研究

A. Cali
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引用次数: 0

摘要

本研究从发酵骆驼乳中分离乳酸菌并对其进行鉴定,并对其作为益生菌剂的能力进行筛选。本研究收集了12份骆驼奶样品,在MRS和M17选择性培养基上分离得到40株分离菌株。从MRS培养基中分离到乳杆菌属(Lactobacillus)和Pediococcus两属,根据形态学和生化特征分离到M17培养基、乳球菌属(Lactococcus)和Leuconostoc。根据乳酸菌和乳球菌的糖发酵模式,将其分为2组(A、B组)和3组(A、B、C组),分别为果糖、半乳糖、葡萄糖、麦芽糖、山梨醇、蔗糖和d -木糖。所有分离物对大肠杆菌和金黄色葡萄球菌的抑菌活性进行进一步筛选。根据抑菌带选择9株进行生物系统鉴定,其中血过氧化物肠球菌(Enterococcus haemoperoxidus)和garvieae乳球菌(Lactoccus garvieae)作为实验室菌株。因此,从这些研究结果来看,乳酸菌分离物可以作为对抗某些病原体的生物制剂。今后应进一步研究乳酸菌的益生菌特性,并采用分子和表型技术对乳酸菌进行种类鉴定。
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Isolation and Screening of Lactic Acid Bacteria From Fermented Camel Milk For Their Antimicrobial Activity Against Common Pathogens
The study was undertaken to isolate and character lactic acid bacteria (LAB) from fermented camel milk and to screen their ability as a probiotic agent. In this research, 12 camel milk samples were collected and a total of 40 isolates were isolated on a MRS and M17 selective Medias. From MRS media, two genera belonging to Lactobacillus and Pediococcus were isolated while M17 media, Lactococcus and Leuconostoc were isolated based on their morphological and biochemical characteristics. Lactobacillus and Lactococcus were divided into two (group A and B) and three (group A, B and C) respectively on the basis of their sugar fermentation pattern of fructose, galactose, glucose, maltose, sorbitol, sucrose and D-xylose. All of LAB isolates were further screened for their antimicrobial activity against E. coli and S.aureus. Based on the zone of inhibition, 9 isolates were selected for further identification using Biolog system: Enterococcus haemoperoxidus and Lactoccus garvieae species were found as LABs. Hence, from these findings the LAB isolate can be used as biological agent against some pathogens. Further studies should be conducted to characterize probiotic properties of LAB and molecular and phenotypic techniques should be employed for species identification of LABs.
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