番茄抗花叶病毒基因tm-1的Rapd标记

T. Ohmori, M. Murata, F. Motoyoshi
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引用次数: 5

摘要

利用随机扩增多态性DNA (RAPD)标记,对2个番茄近等基因系(NILs)进行了筛选,其中1个系携带抗番茄花叶病毒(ToMV)的Tm-1基因,另1个系携带该病毒的易感等位基因。在220种不同的10碱基寡核苷酸引物的1030个聚合酶链反应(PCR)产物中,有12个片段在NILs之间存在多态性。从这12个片段中任意选择6个标记,检测它们是否与125株BC1植物中的Tm-1相关。5个标记与Tm-1之间未检测到重组。另一个标记也被证明与Tm-1有关联,但由于很难将RAPD带与相邻的PCR产物区分开来,因此无法确定它们的遗传距离。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
RAPD MARKERS LINKED TO THE TOMATO MOSAIC VIRUS RESISTANCE GENE, TM-1, IN TOMATO
Random amplified polymorphic DNA (RAPD) markers were screened in two nearly isogenic lines (NILs) of tomato, one of which carried Tm-1 gene conferring resistance to tomato mosaic virus (ToMV) and the other carried its susceptible allele. Among 1030 polymerase chain reaction (PCR) products generated by using 220 different 10-base oligonucleotide primers, 12 fragments were polymorphic between the NILs. Six markers arbitrarily chosen from these 12 fragments were examined whether they link to the Tm-1 in 125 BC1 plants. No recombinations were detected between the five markers and the Tm-1. The other one marker was also proved to link to the Tm-1, but their genetic distance was not determined due to some difficulty in distinguishing the RAPD band from the adjacent PCR products.
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