脂联素基因多态性与2型糖尿病和代谢综合征的关系

Rabia Farooq , Sabhiya Majid , Showkat Ahmad Bhat , Shajrul Amin , Mohammad Hayat Bhat , Hilal Ahmad Wani , Parvaiz Ahmad Shah
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引用次数: 5

摘要

糖尿病的流行在当今世界日益受到人们的关注。2型糖尿病是多种遗传变异和环境危险因素复杂相互作用的结果。这种复杂的疾病以各种脂肪因子水平的遗传变化为特征。方法本研究选取年龄在30 ~ 60岁的成年人群,其中T2DM患者400例,MetS患者150例,对照组300例,其中男女比例为2.3。样本取自斯利那加SMHS内分泌科。我们估计了T2DM受试者中各种脂肪因子水平的相关性,我们观察了T2DM和MetS患者中SNP+276和+45多态性与对照组的关联。采用ELISA和RFLP-PCR检测脂肪因子水平,并进行测序。结果脂联素水平明显低于正常人,而瘦素和抵抗素水平明显高于正常人。瘦素和抵抗素与脂联素呈正相关,而与脂联素呈显著负相关。SNP+45基因型和等位基因频率在T2DM、MetS病例和对照组之间无显著差异。对于+276,结果显示,与SNP+45T等位基因相比,SNP+276G等位基因在克什米尔人群中有增加T2DM风险的趋势。T2DM和MetS患者与对照组ADIPOQ SNP +45 (T/G)基因型和等位基因频率无显著差异(基因型P = 0.15;等位基因P = 0.3)。然而,ADIPOQ的SNP +276 (G/T)基因型和等位基因频率与糖尿病和代谢综合征的发生风险显著相关。结论肥胖及其相关并发症可引起2型糖尿病、代谢综合征患者脂肪因子水平的显著变化,且2型糖尿病、代谢综合征与ADIPOQ基因SNP+276 (G/T)多态性存在显著相关性,提示其在克什米尔人群2型糖尿病和代谢综合征中起重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Association of adiponectin gene polymorphism with type 2 diabetes and metabolic syndrome

Background

Diabetes, the current epidemic in the present world is the growing concern. Type 2 diabetes mellitus is a consequence of complex interactions among multiple genetic variants and environmental risk factors. This complex disorder is characterized by genetic changes in various adipokine levels.

Methods

In this study, adult population ranging from (30–60 years) with sample size of 400 T2DM cases, 150 MetS cases and 300 controls, among which female: male ratio was 2.3. Samples were taken from the Department of endocrinology SMHS Srinagar. We estimated the correlation of various adipokine levels among T2DM subjects, and we observe association of SNP+276 and +45 polymorphism among T2DM and MetS cases with control groups. Adipokine levels were estimated by ELISA and polymorphism by RFLP-PCR, followed by sequencing.

Results

Our results suggested that adiponectin levels are found to be significantly lower in cases while as leptin and resistin levels were found to be higher in cases than normals. Leptin and resistin were found to be positively correlated whereas they were found to have significant negative correlation with adiponectin. The genotype and allele frequencies for SNP+45 did not differ significantly between the T2DM, MetS cases and control groups. For +276, the results revealed a trend towards an increased risk of T2DM for the SNP+276G allele as compared with the SNP+45T allele in the Kashmiri population. The genotype and allele frequencies of SNP +45 (T/G) in ADIPOQ were not significantly different between both T2DM and MetS cases and controls (P = 0.15 for genotype; P = 0.3 for allele). However, the genotype and allele frequencies of SNP +276 (G/T) of ADIPOQ was significantly found to be associated with the risk of developing diabetes and metabolic syndrome.

Conclusion

The present study revealed that the adiposity or its related complications involving type 2 diabetes mellitus, metabolic syndrome causes significant changes in adipokine levels, also there is a significant association between type 2 diabetes mellitus, metabolic syndrome and polymorphism in SNP+276 (G/T) of ADIPOQ gene which suggests its important role in type 2 diabetes mellitus and metabolic syndrome of Kashmir population.

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