在covinas, Sucre中首次报道猫(Felis catus)的遗传变异，使用表型标记

Q2 Veterinary

Revue De Medecine Veterinaire Pub Date : 2017-12-11 DOI:10.19052/MV.5169

Enrique Pardo Pérez, María Teresa Martínez Bula, José Darío Zambrano Charrasquiel

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引用次数: 1

摘要

这项研究的目的是在哥伦比亚苏克雷的科维纳斯，利用编码颜色、设计和皮毛长度的基因，确定家猫(Felis catus)种群的遗传变异。2014年9月至12月，在Covenas 5个社区的187只成年动物中进行了随机抽样，每个动物都有表型特征。命名这符合委员会所使用的标准化Genetic Nomenclature(1968年)、和照看autosomicos标记codificacion morfologica:橙色联系资格性(或)和属地autosomicos non-agouti (a), tabby blotched(结核)、long的二恶英(d)、(l)观测怀特(s)和伍德罗·怀特(W)。计算parametros人口geneticos:本研究的目的是评估猫种群间的系统发育关系，以确定它们的等位基因频率、遗传多样性、基因流动、哈迪-温伯格平衡和遗传距离。结果表明，非刺鼠标记物的频率较高，而斑纹斑纹和显性白色基因的频率较低。遗传多样性主要发生在群体内(HS)，群体间(DST)较少，基因流较高。我们观察到种群中杂合子过多。没有哈迪-温伯格平衡。这些种群在基因上是密切相关的。在本研究中，我们分析了两种不同类型的刺鼠(刺鼠、刺鼠、刺鼠和刺鼠)。本研究旨在通过编码哥伦比亚苏克雷Covenas猫毛的颜色、设计和长度的基因，确定家养猫种群(Felis catus)的遗传变异。Random samples是司2014年9月与12月from 187五成人动物in neighborhoods of Covenas,和每一个动物was phenotypically中西文化。The nomenclature用来in this research follows The国内标准化Genetic nomenclature Cat检查(1968年),and The autosomal markers of morphological奥兰治编码:申诉linked to性(或)and The autosomal属地Non-agouti (a), tabby blotched(结核)、long的二恶英(d)、(l)观测怀特(s)和伍德罗·怀特(W)。The Genetic parameters人口计算:allele frequency,遗传多样性,基因flow, Hardy-Weinberg平衡,and Genetic distance;= =地理= =根据美国人口普查，该地区的总面积为，其中土地和(1.664平方公里)水。It was found that the詹姆希德Non-agouti was the most frequent,而tabby blotched和伍德罗·怀特基因最基层的价值观。在被研究的种群中发现了大部分的遗传多样性(HS)，种群间的多样性(DST)较低，基因流动较高。= =地理根据美国人口普查局的数据，该县总面积为，其中土地和(1.7%)水。这是哈迪-温伯格平衡。人口在基因上密切相关。= =地理= =根据美国人口普查，这个县的总面积为，其中土地和(1.664平方公里)水。这项研究的目的是确定在哥伦比亚苏克雷的科维纳斯，家猫(Felis catus)种群的遗传变异，利用编码颜色、设计和皮毛长度的基因。在2014年9月至12月期间，我们对187只成年动物进行了随机抽样，这些动物分布在Covenas的5个社区，每个社区都有表型特征。使用的命名这个呃concordância com或委员会(1968年)、e 44,2002标准化Genetic Nomenclature atende os autossomicos标记的codificacao morfologica:奥兰治联系或资格性(或)和os属地autossomicos Non-agouti (A), tabby blotched(结核)、long的二恶英(d)、(l)观测怀特(s)和伍德罗·怀特(W),几Calcularam-se osâ米geneticos populacionais:等位基因频率、遗传多样性、基因流动、哈迪-温伯格平衡和遗传距离，并推断了猫种群之间的系统发育关系。非刺鼠标记的频率最高，而斑纹斑纹和显性白色基因的频率最低。遗传多样性主要发生在群体内部(HS)，群体间(DST)较少，基因流动较高。在人群中观察到杂合子过多。没有哈迪-温伯格平衡。人口在基因上是密切相关的。此外，还发现了一种可能的自然和人工选择的非刺鼠位点。

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Primer reporte de la variabilidad genética del gato (Felis catus), con marcadores fenotípicos en Coveñas, Sucre

espanolEl objetivo de esta investigacion fue determinar la variabilidad genetica de las poblaciones de gatos domesticos (Felis catus) utilizando genes que codifican la coloracion, el diseno y la longitud del pelaje en Covenas, Sucre, Colombia. Se realizaron muestreos aleatorios entre septiembre y diciembre de 2014, en 187 animales adultos presentes en cinco barrios de Covenas, donde se caracterizo fenotipicamente cada animal. La nomenclatura utilizada esta en concordancia con el Committee Standardized Genetic Nomenclature For Cats (1968), y atiende a los marcadores autosomicos de codificacion morfologica: el locus ligado al sexo Orange (O) y los loci autosomicos non-agouti (a), tabby blotched (Tb), dilution (d), long hair (l) spotting white (s) y dominant white (W). Se calcularon los parametros geneticos poblacionales: frecuencia alelica, diversidad genetica, flujo genico, equilibrio Hardy-Weinberg y distancia genetica, y se infirieron las relaciones filogeneticas entre las poblaciones de gatos. Se encontro que el marcador non-agouti fue el de mayor frecuencia, mientras los genes tabby blotched y dominant white presentaron los valores mas bajos. La mayor parte de la diversidad genetica se encontro dentro de las poblaciones (HS) y poca entre las poblaciones (DST) y un elevado flujo genico. Se observo un exceso de heterocigotos en la poblacion. No hubo equilibrio Hardy-Weinberg. Las poblaciones se encuentran muy relacionadas geneticamente. Ademas, se evidencio una posible seleccion natural y artificial del locus non-agouti. EnglishThis research aimed to determine genetic variability in domestic cat populations (Felis catus) using genes that codify the coloration, design and length of the coat in Covenas, Sucre, Colombia. Random samples were collected between September and December 2014 from 187 adult animals in five neighborhoods of Covenas, and each animal was characterized phenotypically. The nomenclature used in this research follows the Standardized Genetic Nomenclature for the Domestic Cat (1968), and examines the autosomal markers of morphological coding: the locus linked to sex Orange (O) and the autosomal loci Non-agouti (a), tabby blotched (Tb), dilution (d), long hair (l) spotting white (s) and dominant white (W). The genetic parameters of the population were calculated: allele frequency, genetic diversity, gene flow, Hardy-Weinberg equilibrium, and genetic distance; and phylogenetic relationships among cat populations were inferred. It was found that the Non-agouti marker was the most frequent, while the tabby blotched and dominant white genes had the lowest values. Most genetic diversity was found within the studied populations (HS), with little diversity between populations (DST), and high gene flow was evidenced. An excess of heterozygotes was observed in the population. There was no Hardy-Weinberg equilibrium. Populations are genetically closely related. In addition, a possible natural and artificial selection of the Non-agouti locus was evidenced. portuguesO objetivo desta pesquisa foi determinar a variabilidade genetica das populacoes de gatos domesticos (Felis catus) utilizando genes que codificam a coloracao, o desenho e a longitude da pelagem em Covenas, Sucre, na Colombia. Realizaram-se amostras aleatorias entre setembro e dezembro de 2014, em 187 animais adultos presentes em cinco bairros de Covenas, onde se caracterizou fenotipicamente cada animal. A nomenclatura utilizada esta em concordância com o Committee Standardized Genetic Nomenclature For Cats (1968), e atende a os marcadores autossomicos de codificacao morfologica: o locus ligado a sexo Orange (O) e os loci autossomicos Non-agouti (a), tabby blotched (Tb), dilution (d), long hair (l) spotting white (s) e dominant white (W). Calcularam-se os parâmetros geneticos populacionais: frequencia alelica, diversidade genetica, fluxo genico, equilibrio Hardy-Weinberg e distância genetica, e se inferiram as relacoes filogeneticas entre as populacoes de gatos. Constatou-se que o marcador Non-agouti foi o de maior frequencia, e enquanto que os genes tabby blotched e dominant white apresentaram os valores mais baixos. A maior parte da diversidade genetica foi encontrada dentro das populacoes (HS) e pouca entre as populacoes (DST) e um elevado fluxo genico. Observouse um excesso de heterozigotos na populacao. Nao houve equilibrio Hardy-Weinberg. As populacoes encontram-se muito relacionadas geneticamente. Alem do mais, evidenciou- se uma possivel selecao natural e artificial do locus Non-agouti.

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来源期刊

Revue De Medecine Veterinaire 农林科学-兽医学

CiteScore

1.30

自引率

0.00%

发文量

审稿时长

18-36 weeks

期刊介绍： The Revue de Médecine Vétérinaire publishes four kinds of text: 1) Scientific reviews on subjects related to veterinary and comparative medicine. Suggested length: 10 to 30 typed pages. 2) Original reports on fundamental or applied research. Suggested length: 10 to 15 typed pages. 3) Continuous education articles, that should be easily understandable by non-specialists. Suggested length: 10 to 15 typed pages. 4) Clinical reports. Suggested length: 5 to 15 typed pages. The publication can be done in French language or English language. For an article written in English by not english native speakers authors, the manuscript must be subjected by attesting that it was read again by an anglophone scientist or a scientific translator. The authors must certify that the manuscript was not published or subjected for publication to another review. The manuscript must be accompanied by a sheet signed by all the joint authors indicating their agreement for the tender of the manuscript. The publication is free but a financial participation could be required for the photographs color. An estimate will be sent to collect the agreement of the authors.