联合高效液相色谱法纯化分离伊拉克乳香植物甾醇(会议论文)#

Murtadha Saleh Hussien, Ayad A. Al-Hamashi
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引用次数: 1

摘要

传统医学中使用的这些植物之一是山苔树。它属于菊科。它在世界上有各种各样的常见名称,包括带刺生菜、野生生菜、锯齿生菜和Kahu和khas。利用组合式NEXTGEN技术和高效液相色谱(HPLC)技术,分离和鉴定了乳香树(Lactuca serriola)的部分活性成分。用80%乙醇提取,用己烷分馏。将250 mg正己烷萃取物与4 g硅胶混合,装入软骨内,用乙酸乙酯:正己烷(10%乙酸乙酯,90%己烷v/v)组成的溶剂体系与combi flash的金柱结合,洗脱柱。用薄层色谱法和高效液相色谱法对所得馏分进行分析。通过闪柱色谱分离得到白色针状结晶物质。采用薄层色谱法和高效液相色谱法对分离产物进行分析。从250 mg的己烷馏分中得到β-谷甾醇(11 mg)和豆甾醇(7 mg)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Phytosterol Profile in Iraqi Lactuca serriola after Purification and Isolation by Combiflash and HPLC (Conference Paper )#
One of these plants utilized in traditional medicine is Lactuca seriolla Linn., which belongs to the Asteraceae family. It goes by a variety of common names in the world, including prickly lettuce, wild lettuce, jagged lettuce, and Kahu and khas. The work aimed to isolate and characterize some bioactive constituent(s) from the aerial part of Lactuca serriola utilizing  Combiflash NEXTGEN and high-performance liquid chromatography (HPLC). Lactuca serriola (aerial part) was extracted with 80% ethanol, then fractionated with hexane. Then 250 mg of hexane extract was mixed with 4 g of silica gel and loaded in cartilage, then bounded to the gold column of combi flash using a solvent system comprised of ethyl acetate: n-hexane (10% ethyl acetate 90% hexane v/v) to elute the column. The fractions resulting were analyzed by thin layer chromatography and high-performance liquid chromatography (HPLC). white needle-like crystalline substances were isolated via combi flash column chromatography. The isolated product was analyzed by thin layer chromatography and high-performance liquid chromatography (HPLC). β-sitosterol (11 mg) and stigmasterol (7 mg) were obtained from 250 mg of hexane fraction.  
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