基于重组酶聚合酶扩增的快速检测病毒、类病毒和细菌植物病原体的分析系统

http://eng.biomos.ru/conference/articles.htm Pub Date : 2021-01-01 DOI:10.37747/2312-640x-2021-19-242-244

A. Ivanov, A. Zherdev, B. Dzantiev

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引用次数: 0

摘要

结合重组酶聚合酶扩增和膜试纸，开发了植物病原体检测系统。检测系统可检测马铃薯X病毒、马铃薯梭形块茎类病毒、马铃薯黑腿病菌(Dickeya solani)，以及三种病毒的多重分析。在37℃下进行扩增。分析时间不超过30分钟。

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ANALYTICAL SYSTEMS BASED ON RECOMBINASE POLYMERASE AMPLIFICATION FOR RAPID DETECTION OF VIRAL, VIROID AND BACTERIAL PLANT PATHOGENS

Test systems have been developed for the detection of phytopathogens, combining recombinase polymerase amplification and membrane test strips. Test systems provide detection of potato virus X, potato spindle tuber viroid, potato blackleg pathogen (Dickeya solani), as well as multi-analysis of three viruses. Amplification is carried out at 37 °C. The analysis time does n ot exceed 30 min.

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