Isolation and Characterization of Microorganisms that Degrade Dimethoate

Microorganisms have the ability to degrade a large number of pollutants found in the environment. Microorganisms such as bacteria can degrade toxic compounds and chemicals in an environment friendly way. Dimethoate is an organophosphate pesticide. It is a broad-spectrum insecticide. In the present study, dimethoate degrading microorganisms have been isolated from various soil samples. The different isolates that were obtained were characterized by various biochemical tests. Standardization of degradation was performed. Leuco crystal violet reagent was used as an indicator and colorimetric analysis was done at 595 nm. In total, 8 isolates were got. 3 belonged to Bacillus sp. and 3 were identified as Staphylococcus aureus. 2 isolates were identified as Aspergillus sp. HPLC analysis was carried out for the organism that showed the best degradation results.