{"title":"局部分离有益微生物(LIBeM)在不同浓度油泥污染土壤中的生物强化效果:实验室规模","authors":"Z. NurZaida, M. T. Piakong","doi":"10.4172/2155-6199.1000430","DOIUrl":null,"url":null,"abstract":"An aerated static pile ASP-bioreactor system made up of acrylic material dimension (60 cm × 40 cm × 20 cm) was developed to examine the potential of single and microbial consortium of LIBeM to remediate oil sludge contaminated soil at different concentration levels. Three different strains of LIBeM namely P. aeruginosa-BAS-Cr1, S. paucimobilis-ReTOS-Cr1 and S. maltophilia-RAS-Cr1 were used in this study was obtained from Environmental Microbiology Laboratory, Universiti Malaysia Sabah. Five sets of experiment filled with 10 kg of soils contaminated with 5% and 10% of oil sludge were carried out as Treatment 1 (contaminated soil+P. aeruginosa-BAS-Cr1), Treatment 2 (contaminated soil+S. paucimobilis-ReTOS-Cr1), Treatment 3 (contaminated soil+S. maltophilia-RASCr1), Treatment 4 (contaminated soil+Microbial Consortium; P. aeruginosa-BAS-Cr1+S. paucimobilis-ReTOSCr1+ S. maltophilia-RAS-Cr1) and Treatment 5 (contaminated soil+indigenous bacteria in soil; NA). Their ability to degrade hydrocarbon in the soil was investigated during 60 days incubation periods. Physical and chemical analyses were carried out from each of the treatment and control plot on a weekly basis to check for pH, moisture content, temperatures and Total Petroleum Hydrocarbon (TPH). The results showed that single strain P. aeruginosa- BAS-Cr1 has the highest oil degrading capacity compared to microbial consortium with 80% and 85.2% at both concentration studied. The percentage of TPH removal by P. aeruginosa-BAS-Cr1 is 3-fold higher than NA, thus confirmed that the addition of oil selective degrading bacteria was much better than the control plot. High degradation of long chain alkanes were observed between the control and treatment plot suggested that bioaugmentation using single and microbial consortium had decrease the level of oil sludge in contaminated soil.","PeriodicalId":15262,"journal":{"name":"Journal of Bioremediation and Biodegradation","volume":"2013 1","pages":"1-7"},"PeriodicalIF":0.0000,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"13","resultStr":"{\"title\":\"Effectiveness of Single and Microbial Consortium of Locally Isolated Beneficial Microorganisms (LIBeM) in Bioaugmentation of Oil Sludge Contaminated Soil at Different Concentration Levels: A Laboratory Scale\",\"authors\":\"Z. NurZaida, M. T. Piakong\",\"doi\":\"10.4172/2155-6199.1000430\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"An aerated static pile ASP-bioreactor system made up of acrylic material dimension (60 cm × 40 cm × 20 cm) was developed to examine the potential of single and microbial consortium of LIBeM to remediate oil sludge contaminated soil at different concentration levels. Three different strains of LIBeM namely P. aeruginosa-BAS-Cr1, S. paucimobilis-ReTOS-Cr1 and S. maltophilia-RAS-Cr1 were used in this study was obtained from Environmental Microbiology Laboratory, Universiti Malaysia Sabah. Five sets of experiment filled with 10 kg of soils contaminated with 5% and 10% of oil sludge were carried out as Treatment 1 (contaminated soil+P. aeruginosa-BAS-Cr1), Treatment 2 (contaminated soil+S. paucimobilis-ReTOS-Cr1), Treatment 3 (contaminated soil+S. maltophilia-RASCr1), Treatment 4 (contaminated soil+Microbial Consortium; P. aeruginosa-BAS-Cr1+S. paucimobilis-ReTOSCr1+ S. maltophilia-RAS-Cr1) and Treatment 5 (contaminated soil+indigenous bacteria in soil; NA). Their ability to degrade hydrocarbon in the soil was investigated during 60 days incubation periods. Physical and chemical analyses were carried out from each of the treatment and control plot on a weekly basis to check for pH, moisture content, temperatures and Total Petroleum Hydrocarbon (TPH). The results showed that single strain P. aeruginosa- BAS-Cr1 has the highest oil degrading capacity compared to microbial consortium with 80% and 85.2% at both concentration studied. The percentage of TPH removal by P. aeruginosa-BAS-Cr1 is 3-fold higher than NA, thus confirmed that the addition of oil selective degrading bacteria was much better than the control plot. 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引用次数: 13
摘要
采用丙烯酸材料尺寸(60 cm × 40 cm × 20 cm)设计了曝气静态桩式asp -生物反应器系统，考察了LIBeM单体和微生物联合体对不同浓度油泥污染土壤的修复潜力。本研究使用的3株LIBeM分别为铜绿假单胞菌- bas - cr1、少动假单胞菌- reos - cr1和嗜麦单胞菌- ras - cr1，它们来自马来西亚沙巴大学环境微生物学实验室。试验分为5组，分别填充含5%和10%油泥污染土壤10 kg，作为处理1(污染土壤+P)。处理2(污染土壤+S);处理3(污染土壤+S);maltopophilia - rascr1)，处理4(污染土壤+微生物联合体;p . aeruginosa-BAS-Cr1 + S。paucimobilis-ReTOSCr1+ S. maltopophilia - ras - cr1)和处理5(污染土壤+土源细菌);NA)。在60天的潜伏期内，研究了它们降解土壤中碳氢化合物的能力。每周对每个处理和对照地块进行物理和化学分析，以检查pH、水分含量、温度和总石油烃(TPH)。结果表明，单菌株P. aeruginosa- as - cr1在两种浓度下对油脂的降解能力分别为80%和85.2%。P. aeruginosa-BAS-Cr1对TPH的去除率是NA的3倍，证实了油选择性降解菌的添加效果明显优于对照。长链烷烃在对照区和处理区均有较高的降解率，表明单菌种和微生物联合体的生物强化处理降低了污染土壤中油泥的含量。
Effectiveness of Single and Microbial Consortium of Locally Isolated Beneficial Microorganisms (LIBeM) in Bioaugmentation of Oil Sludge Contaminated Soil at Different Concentration Levels: A Laboratory Scale
An aerated static pile ASP-bioreactor system made up of acrylic material dimension (60 cm × 40 cm × 20 cm) was developed to examine the potential of single and microbial consortium of LIBeM to remediate oil sludge contaminated soil at different concentration levels. Three different strains of LIBeM namely P. aeruginosa-BAS-Cr1, S. paucimobilis-ReTOS-Cr1 and S. maltophilia-RAS-Cr1 were used in this study was obtained from Environmental Microbiology Laboratory, Universiti Malaysia Sabah. Five sets of experiment filled with 10 kg of soils contaminated with 5% and 10% of oil sludge were carried out as Treatment 1 (contaminated soil+P. aeruginosa-BAS-Cr1), Treatment 2 (contaminated soil+S. paucimobilis-ReTOS-Cr1), Treatment 3 (contaminated soil+S. maltophilia-RASCr1), Treatment 4 (contaminated soil+Microbial Consortium; P. aeruginosa-BAS-Cr1+S. paucimobilis-ReTOSCr1+ S. maltophilia-RAS-Cr1) and Treatment 5 (contaminated soil+indigenous bacteria in soil; NA). Their ability to degrade hydrocarbon in the soil was investigated during 60 days incubation periods. Physical and chemical analyses were carried out from each of the treatment and control plot on a weekly basis to check for pH, moisture content, temperatures and Total Petroleum Hydrocarbon (TPH). The results showed that single strain P. aeruginosa- BAS-Cr1 has the highest oil degrading capacity compared to microbial consortium with 80% and 85.2% at both concentration studied. The percentage of TPH removal by P. aeruginosa-BAS-Cr1 is 3-fold higher than NA, thus confirmed that the addition of oil selective degrading bacteria was much better than the control plot. High degradation of long chain alkanes were observed between the control and treatment plot suggested that bioaugmentation using single and microbial consortium had decrease the level of oil sludge in contaminated soil.
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