Penetration of Dialysis Tubes to Components Absorbed in the Plasma of the Extract of Fructus Evodiae: Methodology of the Preparation of Herbal Extract for Pharmacological Experiment in Vitro

Dialysis tubes (Mw 100 D and Mw 500 D) were used to dialyze and purify the water extraction of Wuzhuyu (Fructus Evodiae) and Wuzhuyu decoction, and three parts of molecular weight less than 100 D, between 100 and 500 D, and more than 500 D were obtained. To ascertain the peak time point efficiency, several chemicals treated at different time periods by high performance liquid chromatography (HPLC) were analyzed, and the activity of blood vessels was studied in vitro. By comparing different dialyzing times for three chemicals including evodiamine, isorhamnetin, and limonin in 100–500 D, the best treatment time was found to be 48 h. The portion more than 100 D significantly contracted blood vessels, while the portion less than 100 D did not. In conclusion, it may be feasible to dispose samples using dialysis tubes with different apertures and apply the extract to pharmacological evaluation in vitro.