微尺度狭窄微流体血栓模型表征的计算流体动力学模拟

Q4 Biochemistry, Genetics and Molecular Biology
Y. Zhao, Parham Vatankhah, Tiffany Goh, Jiaqiu Wang, Xuanyi Chen, M. N. Kashani, Keke Zheng, Zhiyong Li, L. Ju
{"title":"微尺度狭窄微流体血栓模型表征的计算流体动力学模拟","authors":"Y. Zhao, Parham Vatankhah, Tiffany Goh, Jiaqiu Wang, Xuanyi Chen, M. N. Kashani, Keke Zheng, Zhiyong Li, L. Ju","doi":"10.32604/MCB.2021.012598","DOIUrl":null,"url":null,"abstract":"Platelet aggregation plays a central role in pathological thrombosis, preventing healthy physiological blood fl ow within the circulatory system. For decades, it was believed that platelet aggregation was primarily driven by solu-ble agonists such as thrombin, adenosine diphosphate and thromboxane A2. However, recent experimental fi nd-ings have unveiled an intriguing but complementary biomechanical mechanism — the shear rate gradients generated from fl ow disturbance occurring at sites of blood vessel narrowing, otherwise known as stenosis, may rapidly trigger platelet recruitment and subsequent aggregation. In our Nature Materials 2019 paper [1], we employed micro fl uidic devices which incorporated micro-scale stenoses to elucidate the molecular insights underlying the prothrombotic effect of blood fl ow disturbance. Nevertheless, the rheological mechanisms associated with this stenotic micro fl uidic device are poorly characterized. To this end, we developed a computational fl uid dynamics (CFD) simulation approach to systematically analyze the hemodynamic in fl uence of bulk fl ow mechanics and fl ow medium. Grid sensitivity studies were performed to ensure accurate and reliable results. Interestingly, the peak shear rate was signi fi cantly reduced with the device thickness, suggesting that fabrication of micro fl uidic devices should retain thicknesses greater than 50 µm to avoid unexpected hemodynamic aberra-tion, despite thicker devices raising the cost of materials and processing time of photolithography. Overall, as many groups in the fi eld have designed micro fl uidic devices to recapitulate the effect of shear rate gradients and investigate platelet aggregation, our numerical simulation study serves as a guideline for rigorous design and fabrication of micro fl uidic thrombosis models. blood fl ow rendering was colored by shear rate at constant viscosity. Note the shear rate maxima occurs at the stenosis apex and near the wall while the center forms a low shear pocket. (B and F) Peak shear rate γ max linearly correlates with input bulk shear rate γ 0 for both eccentric and concentric stenoses respectively. Note the concentric stenosis geometry demonstrates better linearity. The shear rate γ (C and G) and shear rate gradient γ ’ (D and H) distribution were analyzed along a sample streamline 1µm above stenosis apex spanning the shear acceleration ( x = − 100 – 0 µm) and deceleration ( x = 0 – 100 µm) zones. Note the γ and γ ’ present equal-space increment in regard to γ 0 . The γ max is located at x = 0 µm while the γ ’ max is located at x = − 5 µm","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"6","resultStr":"{\"title\":\"Computational Fluid Dynamics Simulations at Micro-Scale Stenosis for Microfluidic Thrombosis Model Characterization\",\"authors\":\"Y. Zhao, Parham Vatankhah, Tiffany Goh, Jiaqiu Wang, Xuanyi Chen, M. N. Kashani, Keke Zheng, Zhiyong Li, L. Ju\",\"doi\":\"10.32604/MCB.2021.012598\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Platelet aggregation plays a central role in pathological thrombosis, preventing healthy physiological blood fl ow within the circulatory system. For decades, it was believed that platelet aggregation was primarily driven by solu-ble agonists such as thrombin, adenosine diphosphate and thromboxane A2. However, recent experimental fi nd-ings have unveiled an intriguing but complementary biomechanical mechanism — the shear rate gradients generated from fl ow disturbance occurring at sites of blood vessel narrowing, otherwise known as stenosis, may rapidly trigger platelet recruitment and subsequent aggregation. In our Nature Materials 2019 paper [1], we employed micro fl uidic devices which incorporated micro-scale stenoses to elucidate the molecular insights underlying the prothrombotic effect of blood fl ow disturbance. Nevertheless, the rheological mechanisms associated with this stenotic micro fl uidic device are poorly characterized. To this end, we developed a computational fl uid dynamics (CFD) simulation approach to systematically analyze the hemodynamic in fl uence of bulk fl ow mechanics and fl ow medium. Grid sensitivity studies were performed to ensure accurate and reliable results. Interestingly, the peak shear rate was signi fi cantly reduced with the device thickness, suggesting that fabrication of micro fl uidic devices should retain thicknesses greater than 50 µm to avoid unexpected hemodynamic aberra-tion, despite thicker devices raising the cost of materials and processing time of photolithography. Overall, as many groups in the fi eld have designed micro fl uidic devices to recapitulate the effect of shear rate gradients and investigate platelet aggregation, our numerical simulation study serves as a guideline for rigorous design and fabrication of micro fl uidic thrombosis models. blood fl ow rendering was colored by shear rate at constant viscosity. Note the shear rate maxima occurs at the stenosis apex and near the wall while the center forms a low shear pocket. (B and F) Peak shear rate γ max linearly correlates with input bulk shear rate γ 0 for both eccentric and concentric stenoses respectively. Note the concentric stenosis geometry demonstrates better linearity. The shear rate γ (C and G) and shear rate gradient γ ’ (D and H) distribution were analyzed along a sample streamline 1µm above stenosis apex spanning the shear acceleration ( x = − 100 – 0 µm) and deceleration ( x = 0 – 100 µm) zones. Note the γ and γ ’ present equal-space increment in regard to γ 0 . The γ max is located at x = 0 µm while the γ ’ max is located at x = − 5 µm\",\"PeriodicalId\":48719,\"journal\":{\"name\":\"Molecular & Cellular Biomechanics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular & Cellular Biomechanics\",\"FirstCategoryId\":\"1087\",\"ListUrlMain\":\"https://doi.org/10.32604/MCB.2021.012598\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Biochemistry, Genetics and Molecular Biology\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular & Cellular Biomechanics","FirstCategoryId":"1087","ListUrlMain":"https://doi.org/10.32604/MCB.2021.012598","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 6

摘要

血小板聚集在病理性血栓形成中起核心作用,阻止循环系统内健康的生理血液流动。几十年来,人们一直认为血小板聚集主要是由溶性激动剂如凝血酶、二磷酸腺苷和血栓素A2驱动的。然而,最近的实验发现揭示了一个有趣但互补的生物力学机制——在血管狭窄(或称为狭窄)部位发生的血流干扰产生的剪切速率梯度可能迅速触发血小板募集和随后的聚集。在Nature Materials 2019年发表的论文[1]中,我们采用了包含微尺度狭窄的微流体装置来阐明血流紊乱的血栓形成效应的分子基础。然而,与这种狭窄的微流体装置相关的流变机制尚不清楚。为此,我们建立了一种计算流体动力学(CFD)模拟方法,系统地分析了流体力学和流体介质对流体动力学的影响。进行网格敏感性研究以确保结果准确可靠。有趣的是,峰值剪切速率随着器件厚度的增加而显著降低,这表明尽管较厚的器件增加了材料成本和光刻加工时间,但微流体器件的制造应保持大于50µm的厚度,以避免意外的血流动力学像差。总的来说,由于该领域的许多研究小组已经设计了微流体装置来重现剪切速率梯度的影响并研究血小板聚集,我们的数值模拟研究为严格设计和制造微流体血栓形成模型提供了指导。血流图以恒定粘度下的剪切速率着色。注意,剪切速率最大值出现在狭窄顶点和管壁附近,而中心形成一个低剪切袋。(B和F)偏心和同心狭窄体的峰值剪切率γ max分别与输入体剪切率γ 0线性相关。注意同心狭窄几何表现出更好的线性。剪切速率γ (C和G)和剪切速率梯度γ′(D和H)沿狭窄顶点上方1µm的样品流线分布,跨越剪切加速(x = - 100µm)和减速(x = 0 - 100µm)区域。注意,γ和γ′相对于γ 0呈现等距增量。γ最大值位于x = 0µm,而γ′最大值位于x =−5µm
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Computational Fluid Dynamics Simulations at Micro-Scale Stenosis for Microfluidic Thrombosis Model Characterization
Platelet aggregation plays a central role in pathological thrombosis, preventing healthy physiological blood fl ow within the circulatory system. For decades, it was believed that platelet aggregation was primarily driven by solu-ble agonists such as thrombin, adenosine diphosphate and thromboxane A2. However, recent experimental fi nd-ings have unveiled an intriguing but complementary biomechanical mechanism — the shear rate gradients generated from fl ow disturbance occurring at sites of blood vessel narrowing, otherwise known as stenosis, may rapidly trigger platelet recruitment and subsequent aggregation. In our Nature Materials 2019 paper [1], we employed micro fl uidic devices which incorporated micro-scale stenoses to elucidate the molecular insights underlying the prothrombotic effect of blood fl ow disturbance. Nevertheless, the rheological mechanisms associated with this stenotic micro fl uidic device are poorly characterized. To this end, we developed a computational fl uid dynamics (CFD) simulation approach to systematically analyze the hemodynamic in fl uence of bulk fl ow mechanics and fl ow medium. Grid sensitivity studies were performed to ensure accurate and reliable results. Interestingly, the peak shear rate was signi fi cantly reduced with the device thickness, suggesting that fabrication of micro fl uidic devices should retain thicknesses greater than 50 µm to avoid unexpected hemodynamic aberra-tion, despite thicker devices raising the cost of materials and processing time of photolithography. Overall, as many groups in the fi eld have designed micro fl uidic devices to recapitulate the effect of shear rate gradients and investigate platelet aggregation, our numerical simulation study serves as a guideline for rigorous design and fabrication of micro fl uidic thrombosis models. blood fl ow rendering was colored by shear rate at constant viscosity. Note the shear rate maxima occurs at the stenosis apex and near the wall while the center forms a low shear pocket. (B and F) Peak shear rate γ max linearly correlates with input bulk shear rate γ 0 for both eccentric and concentric stenoses respectively. Note the concentric stenosis geometry demonstrates better linearity. The shear rate γ (C and G) and shear rate gradient γ ’ (D and H) distribution were analyzed along a sample streamline 1µm above stenosis apex spanning the shear acceleration ( x = − 100 – 0 µm) and deceleration ( x = 0 – 100 µm) zones. Note the γ and γ ’ present equal-space increment in regard to γ 0 . The γ max is located at x = 0 µm while the γ ’ max is located at x = − 5 µm
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Molecular & Cellular Biomechanics
Molecular & Cellular Biomechanics CELL BIOLOGYENGINEERING, BIOMEDICAL&-ENGINEERING, BIOMEDICAL
CiteScore
1.70
自引率
0.00%
发文量
21
期刊介绍: The field of biomechanics concerns with motion, deformation, and forces in biological systems. With the explosive progress in molecular biology, genomic engineering, bioimaging, and nanotechnology, there will be an ever-increasing generation of knowledge and information concerning the mechanobiology of genes, proteins, cells, tissues, and organs. Such information will bring new diagnostic tools, new therapeutic approaches, and new knowledge on ourselves and our interactions with our environment. It becomes apparent that biomechanics focusing on molecules, cells as well as tissues and organs is an important aspect of modern biomedical sciences. The aims of this journal are to facilitate the studies of the mechanics of biomolecules (including proteins, genes, cytoskeletons, etc.), cells (and their interactions with extracellular matrix), tissues and organs, the development of relevant advanced mathematical methods, and the discovery of biological secrets. As science concerns only with relative truth, we seek ideas that are state-of-the-art, which may be controversial, but stimulate and promote new ideas, new techniques, and new applications.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信