空气暴露下太平洋牡蛎长牡蛎能量代谢转化及内质网应激调节

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Changhao Gong, Chao Liu, Huan Li, M. Li, Zhaoqun Liu, Weilin Wang, Liyan Wang, L. Song
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引用次数: 6

摘要

太平洋牡蛎长牡蛎是生活在潮间带的重要物种。研究牡蛎对空气暴露的适应机制具有重要意义。本研究采用加权基因共表达网络分析(WGCNA),结合鳃和内收肌的转录组数据,研究了gigas在空气暴露下的代谢转化。暴露于空气5天、7天和9天的牡蛎内收肌中特异性表达的氧化石墨烯模块富集,揭示了呼吸链、蛋白质转换和脂质代谢的阶段性表达,表明能量代谢的转化。在空气暴露过程中,肌肉中的“呼吸链”和“核糖体生物发生”在第5天富集,表明糖原代谢在空气暴露早期阶段占主导地位。在第7天,蛋白质水解调控的许多项被富集,说明碳水化合物不能满足牡蛎内收肌的代谢需要,蛋白质开始被降解以供给能量。与脂质代谢相关的过程在第9天富集。巨螺杆藻极高的糖原含量使其能够以保守的补偿策略长时间维持基本的代谢活动。鳃内空气暴露敏感模组的GO和KEGG富集主要参与“对内质网应激的响应”、“内质网对高尔基转运囊泡膜的响应”和“内质网蛋白质加工”。结果表明,牡蛎对空气暴露的适应机制是一个复杂的调控网络。Hub基因网络和PPI网络分析发现,一些含有锌指结构域的转录因子调节了应激适应的生化反应,表明内质网作为一种对外部应激敏感的调控元件,可以调节空气暴露条件下gigas鳃的凋亡、自噬和蛋白质降解。这些结果将从能量代谢和体内平衡的角度为巨线虫对空气暴露的适应提供新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The transformation of energy metabolism and Endoplasmic Reticulum stress regulation in Pacific oyster Crassostrea gigas under air exposure
The Pacific oyster Crassostrea gigas is an important species living in the intertidal zones. It is of great significance to study the mechanism of oysters to adapt air exposure. In the present study, weighted gene co-expression network analysis (WGCNA) with the transcriptome data of gills and adductor muscle was conducted to investigate the metabolic transformation of C. gigas under air exposure. GO enrichment of modules specifically expressed in adductor muscle of oysters exposed to air for five, seven and nine days revealed the phased expression of respiratory chain, protein turnover and lipid metabolism, indicating the conversion of energy metabolism. During air exposure, “respiratory chain” and “ribosome biogenesis” were enriched in the muscle on the fifth day, suggesting that glycogen metabolism was dominant in the early stages of air exposure. On the seventh day, many terms about the regulation of proteolysis were enriched, indicating that carbohydrates were not be able to meet the metabolic needs in the oyster adductor muscle, and proteins began to be degraded for energy supply. The processes related to lipid metabolism were enriched on the ninth day. The extremely high glycogen content of C. gigas allowed it to maintain a basic metabolic activity for a long time with a conservative compensation strategy. GO and KEGG enrichments of the modules sensitive to air exposure in gills were mainly involved in “response to endoplasmic reticulum stress”, “Endoplasmic Reticulum (ER) to Golgi transport vesicle membrane” and “protein processing in endoplasmic reticulum”. It revealed that the mechanism of oyster adapting to air exposure was a complex regulatory network depending on the ER. Hub gene network and PPI network analyses found that some transcription factors containing zinc finger domains regulated the biochemical reactions for stress adaptation, indicating that the ER, as a regulatory element sensitive to external stress, could regulate apoptosis, autophagy and protein degradation in gills of C. gigas under air exposure. These results would provide new insights into the adaptation of C. gigas to air exposure in terms of energy metabolism and homeostasis.
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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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