斑马鱼组织特异性bmp2b维持中节点依赖性抑制和wnt依赖性激活的协调参与。

Yu Xue, Cencan Xing, Wenjuan Zhang, Can-bin Chen, Jingjin Xu, A. Meng, Yutian Pan
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引用次数: 2

摘要

一个脊椎动物信号中心,在斑马鱼中被称为组织者,对轴的模式和形成至关重要,并受到多种细胞信号通路的调节,包括Wnt, Nodal和Bmp。组织者特异性Bmp2b在维持Bmp活性梯度和背腹模式中起重要作用。然而,目前尚不清楚组织者中bmp2b的转录是如何被调控的。在这项研究中,我们产生了一个bmp2b转基因系Tsg(-2.272bmp2b:gfp),可以复制组织特异性的bmp2b表达。解剖分析显示,一个0.273 kb的最小启动子对于bmp2b在背组织中的表达是必不可少的。报告者实验表明,在胚胎和细胞系中,组织者特异性的bmp2b受Nodal信号的负调控,而受Wnt信号的正调控。启动子分析和染色质免疫沉淀(ChIP)表明,在0.273 kb的启动子中存在一个一致的smad结合元件(SBE) (CAGAC)和一个Lef/ tcf结合元件(LBE) (AGATAA),可以直接与Smad2和β-catenin蛋白结合。总之,这些结果表明,组织特异性bmp2b表达的维持涉及Nodal和Wnt信号的相反和一致的调控。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Coordinate involvement of Nodal-dependent inhibition and Wnt-dependent activation in the maintenance of organizer-specific bmp2b in zebrafish.
A vertebrate signaling center, known in zebrafish as the organizer, is essential for axis patterning and formation and is regulated by multiple cell signaling pathways, including Wnt, Nodal, and Bmp. Organizer-specific Bmp2b plays important roles in the maintenance of the Bmp activity gradient and dorsal-ventral patterning. However, it is unknown how transcription of bmp2b in the organizer is regulated. In this study, we generated a bmp2b transgenic line Tsg(-2.272bmp2b:gfp) that reproduced organizer-specific bmp2b expression. Dissection analysis revealed that a 0.273-kb minimal promoter was indispensable for bmp2b expression in the dorsal organizer. Reporter assays showed that organizer-specific bmp2b is negatively regulated by the Nodal signal and positively regulated by the Wnt signal in both embryos and cell lines. Promoter analysis and chromatin-immunoprecipitation (ChIP) indicated that one consensus Smad-binding element (SBE) (CAGAC) and one Lef/Tcf-binding element (LBE) (AGATAA) were present in the 0.273-kb promoter, and could be directly bound by Smad2 and β-catenin proteins. Together, these results suggest that maintenance of organizer-specific bmp2b expression involves opposite and concerted regulation by Nodal and Wnt signaling.
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